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|Tested species reactivity||Bovine, Chicken, Human, Mouse, Clawed frog|
|Published species reactivity||Yeast, Rabbit, Nematode, Rat, Fruit fly, Human, Mouse, Rhesus monkey|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified bovine ubiquitin conjugated to carrier protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunofluorescence (IF)||2-5 ug/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 14 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
13-1600 has been successfully used in western blot analysis of ubiquitinated proteins in mouse thymocytes.
Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications. The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. The first reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate. .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CSN6 drives carcinogenesis by positively regulating Myc stability.
13-1600 was used in western blot to identify the mechanistic link between Cullin neddylation and Myc ubiquitination/degradation
|Chen J,Shin JH,Zhao R,Phan L,Wang H,Xue Y,Post SM,Ho Choi H,Chen JS,Wang E,Zhou Z,Tseng C,Gully C,Velazquez-Torres G,Fuentes-Mattei E,Yeung G,Qiao Y,Chou PC,Su CH,Hsieh YC,Hsu SL,Ohshiro K,Shaikenov T,Wang H,Yeung SC,Lee MH||Nature communications (5:null)||2014|
Monoclonal antibody against the ectodomain of E-cadherin (DECMA-1) suppresses breast carcinogenesis: involvement of the HER/PI3K/Akt/mTOR and IAP pathways.
13-1600 was used in western blot to test if targeting the ectodomain of E-cadherin is an effective treatment of HER2-positive breast cancers.
|Brouxhon SM,Kyrkanides S,Teng X,Raja V,O'Banion MK,Clarke R,Byers S,Silberfeld A,Tornos C,Ma L||Clinical cancer research : an official journal of the American Association for Cancer Research (19:3234)||2013|
Analysis of nuclear factor-κB (NF-κB) essential modulator (NEMO) binding to linear and lysine-linked ubiquitin chains and its role in the activation of NF-κB.
13-1600 was used in western blot to elucidate NEMO binding to linear ubiquitin chains of different lengths.
|Kensche T,Tokunaga F,Ikeda F,Goto E,Iwai K,Dikic I||The Journal of biological chemistry (287:23626)||2012|
The NER protein Rad33 shows functional homology to human Centrin2 and is involved in modification of Rad4.
13-1600 was used in western blot to study the relationship between Rad33 and the Rad4-Rad23 during DNA repair
|den Dulk B,van Eijk P,de Ruijter M,Brandsma JA,Brouwer J||DNA repair (7:858)||2008|
|Mouse||Not Cited||RhoB facilitates c-Myc turnover by supporting efficient nuclear accumulation of GSK-3.||Huang M,Kamasani U,Prendergast GC||Oncogene (25:1281)||2006|
|Mouse||Not Cited||Activation of the E3 ubiquitin ligase Itch through a phosphorylation-induced conformational change.||Gallagher E,Gao M,Liu YC,Karin M||Proceedings of the National Academy of Sciences of the United States of America (103:1717)||2006|
|Nematode||1:1000||Similar patterns of mitochondrial vulnerability and rescue induced by genetic modification of alpha-synuclein, parkin, and DJ-1 in Caenorhabditis elegans.||Ved R,Saha S,Westlund B,Perier C,Burnam L,Sluder A,Hoener M,Rodrigues CM,Alfonso A,Steer C,Liu L,Przedborski S,Wolozin B||The Journal of biological chemistry (280:42655)||2005|
|Mouse||Not Cited||Involvement of the p97-Ufd1-Npl4 complex in the regulated endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors.||Alzayady KJ,Panning MM,Kelley GG,Wojcikiewicz RJ||The Journal of biological chemistry (280:34530)||2005|
|Human||Not Cited||Methylation silencing of SOCS-3 promotes cell growth and migration by enhancing JAK/STAT and FAK signalings in human hepatocellular carcinoma.||Niwa Y,Kanda H,Shikauchi Y,Saiura A,Matsubara K,Kitagawa T,Yamamoto J,Kubo T,Yoshikawa H||Oncogene (24:6406)||2005|
|Defining the role of ubiquitin-interacting motifs in the polyglutamine disease protein, ataxin-3.||Berke SJ,Chai Y,Marrs GL,Wen H,Paulson HL||The Journal of biological chemistry (280:32026)||2005|
|Human||Not Cited||Role of p97 AAA-ATPase in the retrotranslocation of the cholera toxin A1 chain, a non-ubiquitinated substrate.||Kothe M,Ye Y,Wagner JS,De Luca HE,Kern E,Rapoport TA,Lencer WI||The Journal of biological chemistry (280:28127)||2005|
Interferon-gamma-mediated activation and ubiquitin-proteasome-dependent degradation of PPARgamma in adipocytes.
13-1600 was used in western blot to show that IFNgamma treatment results in increased targeting of PPARgamma to the ubiquitin-proteasome system.
|Floyd ZE,Stephens JM||The Journal of biological chemistry (277:4062)||2002|
Cancer-predisposing mutations within the RING domain of BRCA1: loss of ubiquitin protein ligase activity and protection from radiation hypersensitivity.
13-1600 was used in western blot to report that BRCA has ubiquitin protein ligase activity and that cancer-predisposing mutations within the BRCA RING domain abolish its ubiquitin ligase activity.
|Ruffner H,Joazeiro CA,Hemmati D,Hunter T,Verma IM||Proceedings of the National Academy of Sciences of the United States of America (98:5134)||2001|
|Rhesus monkey||Not Cited||
Proteasomes regulate erythropoietin receptor and signal transducer and activator of transcription 5 (STAT5) activation. Possible involvement of the ubiquitinated Cis protein.
13-1600 was used in western blot to study the relationship between ubiquitinated Cis and the erythropoietin receptor.
|Verdier F,Chrétien S,Muller O,Varlet P,Yoshimura A,Gisselbrecht S,Lacombe C,Mayeux P||The Journal of biological chemistry (273:28185)||1998|
|Mouse||Not Cited||Mitochondrial division ensures the survival of postmitotic neurons by suppressing oxidative damage.||Kageyama Y,Zhang Z,Roda R,Fukaya M,Wakabayashi J,Wakabayashi N,Kensler TW,Reddy PH,Iijima M,Sesaki H||The Journal of cell biology (197:535)||2012|
Optineurin is colocalized with ubiquitin in Marinesco bodies.
13-1600 was used in immunohistochemistry to show that optineurin colocalizes with ubiquitin in Marinesco bodies.
|Schwab C,Yu S,McGeer PL||Acta neuropathologica (123:289)||2012|
Gene expression levels assessed by CA1 pyramidal neuron and regional hippocampal dissections in Alzheimer's disease.
13-1600 was used in immunohistochemistry to evaluate molecular signatures of an individual cell types in patients with of Alzheimer's disease.
|Ginsberg SD,Alldred MJ,Che S||Neurobiology of disease (45:99)||2012|
Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy.
13-1600 was used in immunohistochemistry to explore the role of autophaghy in a Drosophila model of Dentatorubral-pallidoluysian Atrophy
|Nisoli I,Chauvin JP,Napoletano F,Calamita P,Zanin V,Fanto M,Charroux B||Cell death and differentiation (17:1577)||2010|
|Mouse||Not Cited||Early-onset and robust amyloid pathology in a new homozygous mouse model of Alzheimer's disease.||Willuweit A,Velden J,Godemann R,Manook A,Jetzek F,Tintrup H,Kauselmann G,Zevnik B,Henriksen G,Drzezga A,Pohlner J,Schoor M,Kemp JA,von der Kammer H||PloS one (4:null)||2009|
|Human||1:10||Histomathematical analysis of clinical specimens: challenges and progress.||Gannot G,Gillespie JW,Chuaqui RF,Tangrea MA,Linehan WM,Emmert-Buck MR||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (53:177)||2005|
|Rat||1:400||Proteasome mediates dopaminergic neuronal degeneration, and its inhibition causes alpha-synuclein inclusions.||Sawada H,Kohno R,Kihara T,Izumi Y,Sakka N,Ibi M,Nakanishi M,Nakamizo T,Yamakawa K,Shibasaki H,Yamamoto N,Akaike A,Inden M,Kitamura Y,Taniguchi T,Shimohama S||The Journal of biological chemistry (279:10710)||2004|
The Ras-specific exchange factors mouse Sos1 (mSos1) and mSos2 are regulated differently: mSos2 contains ubiquitination signals absent in mSos1.
13-1600 was used in immunoprecipitation to characterize mouse msos and msos2.
|Nielsen KH,Papageorge AG,Vass WC,Willumsen BM,Lowy DR||Molecular and cellular biology (17:7132)||1997|
hPLIC-2; HRIHFB2157; Nedd4 binding protein 4; PLIC-2; protein linking IAP with cytoskeleton 2; Protein linking IAP with cytoskeleton-2; Ubiquilin 2; ubiquilin-2; ubiquitin-like product Chap1/Dsk2; Ubiquitin-related protein
ALS15; CHAP1; DSK2; HRIHFB2157; N4BP4; Plic-2; PLIC2; UBQLN2