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Immunofluorescent analysis of Ubiquitin (green) in HeLa cells either left untreated (left panel) or treated with 50uM of MG132 proteasome inhibitor (right panel) for 90 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Ubiquitin monoclonal antibody (Product # MA1-10035) at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Bovine, C. elegans, Chicken, Fruit fly, Horse, Human, Mouse, Pig, Rat|
|Published species reactivity||Fruit fly, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified ubiquitin conjugated with glutaroldehyde to KLH|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:5000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:5000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has an important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalantly coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.
MA1-10035 was used in western blot to develop a Drosophila model for Charcot-Marie-Tooth by mutating glycyl-tRNA synthetase
|Niehues S,Bussmann J,Steffes G,Erdmann I,Köhrer C,Sun L,Wagner M,Schäfer K,Wang G,Koerdt SN,Stum M,Jaiswal S,RajBhandary UL,Thomas U,Aberle H,Burgess RW,Yang XL,Dieterich D,Storkebaum E||Nature communications (6:null)||2015|
Protein aggregation of SERCA2 mutants associated with Darier disease elicits ER stress and apoptosis in keratinocytes.
MA1-10035 was used in western blot to study the effect of aggregation of SERCA2 mutants associated with Darier disease on ER stress and keratinocyte apoptosis
|Wang Y,Bruce AT,Tu C,Ma K,Zeng L,Zheng P,Liu Y,Liu Y||Journal of cell science (124:3568)||2011|
Laforin is required for the functional activation of malin in endoplasmic reticulum stress resistance in neuronal cells.
MA1-10035 was used in immunocytochemistry to study the role of laforin in the endoplasmic reticulum stress resistance in neuronal cells
|Zeng L,Wang Y,Baba O,Zheng P,Liu Y,Liu Y||The FEBS journal (279:2467)||2012|
40S ribosomal protein S27a; 60S ribosomal protein L40; CG5271-PA; epididymis luminal protein 112; HUBCEP52; male female sterile 31; ribosomal protein S27A; RPL40; RpS27A-PA; Ubiquiti; ubiquitin; Ubiquitin 52 amino acid fusion protein; Ubiquitin A52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 80; ubiquitin fusion 80; ubiquitin-40S ribosomal protein S27a; ubiquitin-CEP80; Ubiquitin-monomeric; ubiquitin/ribosomal protein
0610006J14Rik; BOS_11463; CEP80; CG5271; Dmel\CG5271; Dmel_CG5271; DUb80; HEL112; l(2)04820; l(2)31Ei; mfs(2)31; mfs(2)48; mfs48; RPS27A; S27A; UB3-D; Uba52; UBA80; Ubb; UBC; UBCEP1; UBCEP80; UBI-F80; Ubi-m; Ubiq