Search
Invitrogen
Ubiquitin (linkage specific K27) Recombinant Rabbit Monoclonal Antibody (JE75-30)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 6
Ubiquitin (linkage specific K27) Antibody (MA570070) in ICC/IF
Immunocytochemistry analysis of Ubiquitin (linkage specific K27) in Jurkat cells. Samples were incubated with Ubiquitin (linkage specific K27) monoclonal antibody (Product # MA570070) using a dilution of 1:1,000 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabili... View More
Product Details
MA570070
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE75-30
Immunogen
Synthetic peptide within human ubiquitin aa 20-34.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive control: Jurkat, human spleen tissue, mouse spleen tissue, rat spleen tissue.
Subcellular location: Cell Membrane, Cytoplasmic and Nuclear.
Target Information
Ubiquitin is a small regulatory protein predominantly known for its role in protein degradation via the ubiquitin-proteasome pathway. Post-translational modifications of ubiquitin, such as polyubiquitination, involve linking ubiquitin molecules through one of its seven lysine residues (K6, K11, K27, K29, K33, K48, and K63). The K27-linked ubiquitin chains are of particular interest due to their involvement in non-proteolytic functions, including DNA damage response, transcriptional regulation, and immune response. K27-linked polyubiquitination can signal for the recruitment of specific binding proteins that recognize this distinct linkage, influencing various cellular processes. This specific linkage is structurally distinct from other types of ubiquitin chains, forming a compact and rigid conformation that determines its unique binding properties and functional outcomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: epididymis secretory protein Li 50; polyubiquitin B; Polyubiquitin-B; ubiquitin C; unnamed protein product
Gene Aliases: AL033289; HEL-S-50; Rps27a; Uba52; UBB; Ubb2; UBC
UniProt ID: (Human) P0CG47, (Mouse) P0CG49, (Rat) P0CG51
Entrez Gene ID: (Human) 7314, (Mouse) 22187, (Rat) 192255
male meiosis I
female meiosis I
male gonad development
female gonad development
ubiquitin homeostasis
hypothalamus gonadotrophin-releasing hormone neuron development
mitochondrion transport along microtubule
neuron projection morphogenesis
regulation of mitochondrial membrane potential
adipose tissue development
fat pad development
regulation of proteasomal protein catabolic process
seminiferous tubule development
energy homeostasis
regulation of neuron death
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
protein ubiquitination
modification-dependent protein catabolic process
positive regulation of protein ubiquitination
regulation of neuron apoptotic process
positive regulation of protein monoubiquitination
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support