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|Tested species reactivity||Yeast|
|Published species reactivity||Yeast, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||V-ATPase 60 kDa subunit|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Heme Transport Capacity of LHR1 Determines the Extent of Virulence in Leishmania amazonensis.
A-6427 was used in western blot to study Leishmania LHR1 and heme uptake in Saccharomyces cerevisiae
|Renberg RL,Yuan X,Samuel TK,Miguel DC,Hamza I,Andrews NW,Flannery AR||PLoS neglected tropical diseases (9:null)||2015|
Reducing signs of aging and increasing lifespan by drug synergy.
A-6427 was used in western blot to report a pharmacological strategy that reduces signs of aging in and increases the lifespan of Saccharomyces cerevisiae.
|Huang X,Liu J,Withers BR,Samide AJ,Leggas M,Dickson RC||Aging cell (12:652)||2013|
A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae.
A-6427 was used in western blot to identify the SEA complex is a member of a family of membrane coating and vesicle tethering assemblies needed for intracellular trafficking, amino acid biogenesis, and response to nitrogen starvation.
|Dokudovskaya S,Waharte F,Schlessinger A,Pieper U,Devos DP,Cristea IM,Williams R,Salamero J,Chait BT,Sali A,Field MC,Rout MP,Dargemont C||Molecular & cellular proteomics : MCP (10:null)||2011|
|Not Applicable||Not Cited||
Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p.
A-6427 was used in immunocytochemistry to study the role of Vps35p in transporting cargo proteins.
|Nothwehr SF,Ha SA,Bruinsma P||The Journal of cell biology (151:297)||2000|
|Not Applicable||Not Cited||
The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane.
A-6427 was used in immunocytochemistry to elucidate how the vacuole contributes to iron metabolism.
|Urbanowski JL,Piper RC||The Journal of biological chemistry (274:38061)||1999|