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|Tested species reactivity||Yeast|
|Published species reactivity||Yeast|
|Host / Isotype||Mouse / IgG1|
|Immunogen||V-ATPase 60 kDa subunit|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Heme Transport Capacity of LHR1 Determines the Extent of Virulence in Leishmania amazonensis.
A-6427 was used in western blot to study Leishmania LHR1 and heme uptake in Saccharomyces cerevisiae
|Renberg RL,Yuan X,Samuel TK,Miguel DC,Hamza I,Andrews NW,Flannery AR||PLoS neglected tropical diseases (9:null)||2015|
Reducing signs of aging and increasing lifespan by drug synergy.
A-6427 was used in western blot to report a pharmacological strategy that reduces signs of aging in and increases the lifespan of Saccharomyces cerevisiae.
|Huang X,Liu J,Withers BR,Samide AJ,Leggas M,Dickson RC||Aging cell (12:652)||2013|
A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae.
A-6427 was used in western blot to identify the SEA complex is a member of a family of membrane coating and vesicle tethering assemblies needed for intracellular trafficking, amino acid biogenesis, and response to nitrogen starvation.
|Dokudovskaya S,Waharte F,Schlessinger A,Pieper U,Devos DP,Cristea IM,Williams R,Salamero J,Chait BT,Sali A,Field MC,Rout MP,Dargemont C||Molecular & cellular proteomics : MCP (10:null)||2011|