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V-ATPase C1 Antibody (PA5-117801) in WB
Western Blot using V-ATPase C1 Polyclonal Antibody (Product # PA5-117801) at 1:10,000 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa.
Product Details
PA5-117801
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human ATP6V1C1
Conjugate
Unconjugated
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2902411
Target Information
ATP6V1C1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1; FLJ20057; H(+)-transporting two-sector ATPase, subunit C; H+ -ATPase C subunit; H+-transporting ATPase chain C, vacuolar; subunit C of vacuolar proton-ATPase V1 domain; testicular tissue protein Li 223; V-ATPase C subunit; V-ATPase subunit C 1; V-type proton ATPase subunit C 1; vacuolar ATP synthase subunit C; vacuolar proton pump C subunit; Vacuolar proton pump subunit C 1; vacuolar proton pump, 42-kD subunit; vacuolar proton-ATPase, subunit C, VI domain
Gene Aliases: ATP6C; ATP6D; ATP6V1C1; VATC; Vma5
UniProt ID: (Human) P21283
Entrez Gene ID: (Human) 528
Molecular Function:
ATP synthase
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