Invitrogen

V-ATPase E1 Polyclonal Antibody

Product Image Gallery

V-ATPase E1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of V-ATPase E1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-V-ATPase E1 Rabbit Polyclonal Antibody (Product # PA5-29899) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly Cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

V-ATPase E1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ATP6V1E1 in paraffin-embedded colon carcinoma using an ATP6V1E1 polyclonal antibody (Product # PA5-29899) at a dilution of 1:250.

V-ATPase E1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of U2OS (Lane 1), HeLa (Lane 2), A431 (Lane 3) and HEK 293 (Lane 4). The blot was probed with Anti-V-ATPase E1 Polyclonal Antibody (Product # PA5-29899, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 31 kDa band corresponding to V-ATPase E1 was observed across the cell lines positive for V-ATPase E1.

V-ATPase E1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using V-ATPase E1 polyclonal antibody (Product # PA5-29899) in Immunocytochemistry and Immunohistochemistry

Product Details

PA5-29899

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 200 of Human ATP6V1E1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.96 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2547373

Product Specific Information

Recommended positive controls: A549, H1299, HCT116, mouse brain, rat brain.

Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (82%), Chicken (87%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Macrophages use a bet-hedging strategy for antimicrobial activity in phagolysosomal acidification.

The Journal of clinical investigation

Dragotakes Q,Stouffer KM,Fu MS,Sella Y,Youn C,Yoon OI,De Leon-Rodriguez CM,Freij JB,Bergman A,Casadevall A

PA5-29899 was used in Immunocytochemistry to analyze the dynamical distribution of phagolysosomal pH in murine and human macrophages that had ingested live or dead Cryptococcus neoformans cells, or inert beads.

Wed Jul 01 00:00:00 EDT 2020

Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel.

The Journal of biological chemistry

Shi S,Montalbetti N,Wang X,Rush BM,Marciszyn AL,Baty CJ,Tan RJ,Carattino MD,Kleyman TR

PA5-29899 was used in Immunocytochemistry-Immunofluorescence to investigate the modulatory role of the PON family in in ion channel expression.

Fri Apr 10 00:00:00 EDT 2020

Docosahexaenoic acid inhibits zymogen activation by suppressing vacuolar ATPase activation in cerulein-stimulated pancreatic acinar cells.

Genes & nutrition

Park Y,Ku L,Lim JW,Kim H

Published figure using V-ATPase E1 polyclonal antibody (Product # PA5-29899) in Western Blot

Mon Mar 23 00:00:00 EDT 2020

H⁺-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney.

American journal of physiology. Renal physiology

Frische S,Chambrey R,Trepiccione F,Zamani R,Marcussen N,Alexander RT,Skjødt K,Svenningsen P,Dimke H

Published figure using V-ATPase E1 polyclonal antibody (Product # PA5-29899) in Immunocytochemistry

Sat Sep 01 00:00:00 EDT 2018

H⁺-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney.

American journal of physiology. Renal physiology

Frische S,Chambrey R,Trepiccione F,Zamani R,Marcussen N,Alexander RT,Skjødt K,Svenningsen P,Dimke H

Published figure using V-ATPase E1 polyclonal antibody (Product # PA5-29899) in Immunocytochemistry

Sat Sep 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: ATPase, H+ transporting lysosomal (vacuolar proton pump), 32 kDa; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1; ATPase, H+ transporting, V1 subunit E; H(+)-ATPase E-like protein; H(+)-transporting two-sector ATPase, 31kDa subunit; H+ ATPase subunit E; H+-transporting ATP synthase chain E, vacuolar; lysosomal 31kDa; p31; V-ATPase 31 kDa subunit; V-ATPase subunit E 1; V-ATPase, subunit E; V-type proton ATPase subunit E 1; vacuolar H(+)-ATPase, E subunit; vacuolar H+ ATPase E1; Vacuolar proton pump subunit E 1; VATPase, H+ transporting, lysosomal V1 subunit E1

Gene Aliases: 2410029D23Rik; ATP6E; ATP6E2; ATP6V1E; ATP6V1E1; D6Ertd385e; E2; P31; Vma4

UniProt ID: (Human) P36543, (Mouse) P50518, (Rat) Q6PCU2

Entrez Gene ID: (Human) 529, (Mouse) 11973, (Rat) 297566

Function(s)

transport ion transport ATP hydrolysis coupled proton transport proton transport metabolic process insulin receptor signaling pathway regulation of macroautophagy transferrin transport ion transmembrane transport phagosome acidification

Process(es)

protein binding hydrogen-exporting ATPase activity, phosphorylative mechanism proton-transporting ATPase activity, rotational mechanism ATPase binding hydrolase activity