Immunohistochemistry analysis of VCAM-1 / CD106 (1.4C3) showing staining in the membrane of paraffin-embedded human tonsil tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a VCAM-1 / CD106 Antibody (1.4C3) Mouse Monoclonal Antibody (MA112637) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Stimulated HUVEC cells and mouse myeloma NS1 cells|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Use on formalin-fixed sections requires boiling sections in 1 mM EDTA, pH 8.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes. A suggested positive control for this product is tonsil tissue.
VCAM-1 is distributed across non-leukocyte and leukocyte cells. Its expression is reportedly correlated with adhesion of melanoma, Burkitt and quote;s lymphoma, osteosarcoma, and kidney carcinoma but not colon carcinoma cells to endothelial cells which may determine the site of tumor metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of cell surface expression of endothelial adhesion molecules by ursolic acid prevents intimal hyperplasia of venous bypass grafts in rats.
MA1-12637 was used in immunohistochemistry to study the role of reduced endothelial VCAM-1 expresson in the mechanism by which ursolic acid prevents intimal hyperplasia in a rat model of venous bypass graft
|Zeller I,Wiedemann D,Schwaiger S,Stelzmüller M,Kreutmayer S,Leberfing O,Stuppner H,Bernhard D||European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (42:878)||2012|
Immunophenotypic profile and role of adhesion molecules in splenic marginal zone lymphoma with bone marrow involvement.
MA1-12637 was used in immunohistochemistry to study the distribution and function of adhesion molecules in splenic marginal zone lymphoma
|Florena AM,Tripodo C,Porcasi R,Ingrao S,Fadda MR,De Cantis S,Iannitto E,Franco V||Leukemia and lymphoma (47:49)||2006|
Identification and pharmacological characterization of the anti-inflammatory principal of the leaves of dwarf elder (Sambucus ebulus L.).
MA1-12637 was used in immunocytochemistry to identify and study the active anti-inflammatory component of dwarf elder leaves
|Schwaiger S,Zeller I,Pölzelbauer P,Frotschnig S,Laufer G,Messner B,Pieri V,Stuppner H,Bernhard D||Journal of ethnopharmacology (133:704)||2011|
Leoligin, the major lignan from Edelweiss, inhibits intimal hyperplasia of venous bypass grafts.
MA1-12637 was used in flow cytometry to study the mechanism by which leoligin inhibits intimal hyperplasia of venous bypass grafts
|Reisinger U,Schwaiger S,Zeller I,Messner B,Stigler R,Wiedemann D,Mayr T,Seger C,Schachner T,Dirsch VM,Vollmar AM,Bonatti JO,Stuppner H,Laufer G,Bernhard D||Cardiovascular research (82:542)||2009|