|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 206 of VCP (Uniprot ID#P55072)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-22257 targets VCP(p97) in ICC, IF, IHC (P), and WB applications and shows reactivity with Human, Rat, and mouse samples.
The PA5-22257 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 206 (P55072) of VCP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Diversion of aspartate in ASS1-deficient tumours fosters de novo pyrimidine synthesis.
PA5-22257 was used in western blot to find out the reason ASS1-deficient tumors undergo de novo pyrimidine synthesis without aspartate
|Rabinovich S,Adler L,Yizhak K,Sarver A,Silberman A,Agron S,Stettner N,Sun Q,Brandis A,Helbling D,Korman S,Itzkovitz S,Dimmock D,Ulitsky I,Nagamani SC,Ruppin E,Erez A||Nature (527:379)||2015|