|Tested species reactivity||Human, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human CDH5 (amino acids: 29-223 ) expressed in E. Coli.|
|Storage buffer||PBS with 0.5% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200 - 1:400|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-17050 targets CDH5 in FACS, pep-ELISA, and WB applications and shows reactivity with Human and Rat samples.
The MA5-17050 immunogen is purified recombinant fragment of human CDH5 (amino acids: 29-223 ) expressed in E. Coli.
MA5-17050 detects CDH5 which has a predicted molecular weight of approximately 87.5kDa.
This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Adropin reduces paracellular permeability of rat brain endothelial cells exposed to ischemia-like conditions.
MA5-17050 was used in western blot to study the reduction of paracellular permeability of rat brain endothelial cells exposed to ischemia-like conditions by adropin
|Yang C,DeMars KM,Hawkins KE,Candelario-Jalil E||Peptides (81:29)||2016|