Immunofluorescent analysis of VE-Cadherin (green) in Huvec cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a VE-Cadherin polyclonal antibody (Product # 36-1900) at a dilution of 10ug/ml in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from a C-terminal region of the human VE-cadherin (vascular endothelial cadherin, cadherin-5, CD144) protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||1µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
36-1900 has been successfully used in western blot, immunofluorescence, immunohistochemistry, and ELISA.
This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The loss of sustained Ca(2+) signaling underlies suppressed endothelial nitric oxide production in preeclamptic pregnancies: implications for new therapy.
36-1900 was used in western blot to examine reduced nitric oxide output in preeclampsia subjects.
|Krupp J,Boeldt DS,Yi FX,Grummer MA,Bankowski Anaya HA,Shah DM,Bird IM||American journal of physiology. Heart and circulatory physiology (305:H969)||2013|
|Human||Not Cited||Interference with endothelial cell function by JG-03-14, an agent that binds to the colchicine site on microtubules.||Dalyot-Herman N,Delgado-Lopez F,Gewirtz DA,Gupton JT,Schwartz EL||Biochemical pharmacology (78:1167)||2009|
||Interference with endothelial cell function by JG-03-14, an agent that binds to the colchicine site on microtubules.||Dalyot-Herman N,Delgado-Lopez F,Gewirtz DA,Gupton JT,Schwartz EL||Biochemical pharmacology (78:1167)||2009|