Immunofluorescent staining of MCF-7 cells using PA5-19612, anti-VE Cadherin antibody. The cells were fixed with methanol (100%) for 5 minutes, permabilised with TBS-T (20mins), BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 5 ug/ml overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Chicken, Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human VE Cadherin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunocytochemistry (ICC)||1-5 µg/ml|
|Immunofluorescence (IF)||1-5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Heat mediated antigen retrieval with Tris/EDTA buffer, pH 9.0, recommended prior to tissue staining.
This antibody is predicted to react with cow and pig based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells.
PA5-19612 was used in western blot to analyze the attenuation of the cardiomyogenesis of mouse embryonic stem cells by HIF-1alpha deficiency
|Kudová J,Procházková J,Va¿i¿ek O,Pere¿ko T,Sedlá¿ková M,Pe¿l M,Pacherník J,Kubala L||PloS one (11:null)||2016|