Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.1-0.2 µg/mL | - |
Flow Cytometry (Flow) |
- | View 1 publication 1 publication |
ELISA (ELISA) |
0.25-1.0 µg/mL | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
E.coli-derived, 38.2 kDa Recombinant Human VEGF 165 |
Conjugate |
Biotin |
Form |
Lyophilized |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
-20°C |
AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF 165 (500-P10) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VEGF 165.
Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: MGC70609; RP1-261G23.1; VAS; Vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; Vasculotropin; VEGF-A; VEGF1; VEGFMGC70609; VPF; VPF ant
Gene Aliases: MVCD1; VEGF; VEGFA; VPF
UniProt ID: (Human) P15692
Entrez Gene ID: (Human) 7422
Molecular Function:
growth factor
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