Immunofluorescent analysis of Vascular Endothelial Growth Factor (VEGF, green) in Hela cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with (left panel) or without (right panel) a VEGF polyclonal antibody (Product # MA5-13182) at a dilution of 1:20 for at least 1 hour at room temperature, and then incubated with a Dylight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:1000 for 45 minutes at room temperature. F-actin (both panels, red) was stained by Dylight 554 Phalloidin (Product # 21834) and nuclei (both panels, blue) were stained with DAPI (Product # 46190). Images were taken at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human VEGF from a glioma|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12949 targets Vascular Endothelial Growth Factor in WB and ICC/IF applications and shows reactivity with Human samples.
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of stem cell factor and c-KIT in keloid pathogenesis: do tyrosine kinase inhibitors have a potential therapeutic role?
MA5-12949 was used in western blot to investigate the effect of SCF/c-KIT system on scar pathogenesis
|Mukhopadhyay A,Do DV,Ong CT,Khoo YT,Masilamani J,Chan SY,Vincent AS,Wong PK,Lim CP,Cao X,Lim IJ,Phan TT||The British journal of dermatology (164:372)||2011|
Synergistic effect of celecoxib on 5-fluorouracil-induced apoptosis in hepatocellular carcinoma patients.
MA5-12949 was used in western blot to investigate the therapeutic benefits of cyclooxygenase-2 inhibitor celecoxib in the treatment of hepatocellular carcinoma
|Bassiouny AR,Zaky A,Neenaa HM||Annals of hepatology (9:410)||2010|
In vitro angiogenesis by human umbilical vein endothelial cells (HUVEC) induced by three-dimensional co-culture with glioblastoma cells.
MA5-12949 was used in western blot to evaluate a 3-D in vitro human umbilical vein endothelial cell and glioma co-culture system for angiogenesis study
|Chen Z,Htay A,Dos Santos W,Gillies GT,Fillmore HL,Sholley MM,Broaddus WC||Journal of neuro-oncology (92:121)||2009|
|Non-human primate||Not Cited||
Brain-specific angiogenesis inhibitor 2 regulates VEGF through GABP that acts as a transcriptional repressor.
MA5-12949 was used in western blot to study the mechanism by which brain-specific angiogenesis inhibitor 2 regulates VEGF expression
|Jeong BC,Kim MY,Lee JH,Kee HJ,Kho DH,Han KE,Qian YR,Kim JK,Kim KK||FEBS letters (580:669)||2006|
The molecular mechanism underlying angiogenesis in hepatocellular carcinoma: the imbalance activation of signaling pathways.
MA5-12949 was used in western blot to study the role of VEGF in the angiogenesis in hepatocellular carcinoma
|Zhao ZC,Zheng SS,Wan YL,Jia CK,Xie HY||Hepatobiliary and pancreatic diseases international : HBPD INT (2:529)||2003|
Anti-human vascular endothelial growth factor (VEGF) antibody selection for immunohistochemical staining of proliferating blood vessels.
MA5-12949 was used in immunohistochemistry to evaluate suitable vascular endothelial growth factor antibodies for the detection of proliferating blood vessels
|van der Loos CM,Meijer-Jorna LB,Broekmans ME,Ploegmakers HP,Teeling P,de Boer OJ,van der Wal AC||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (58:109)||2010|