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Immunofluorescence analysis of VEGF was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VEGF (SP28) Rabbit Monoclonal Antibody (MA514573) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from N-terminal of human VEGF|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Ready-to-use|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14573 targets Vascular Endothelial Growth Factor in IHC (P) applications and shows reactivity with Human samples.
The MA5-14573 immunogen is a synthetic peptide derived from N-terminal of human VEGF.
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. This antibody reacts with the 165, 189 and 121 amino acid splice variants of VEGF of human, to a lesser extent, mouse and rat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of bone marrow derived-mesenchymal stem cells in attenuation of kidney function in rats with diabetic nephropathy.
MA5-14573 was used in immunohistochemistry to study the improvement of renal function in a rat model of diabetic nephropathy following treatment with mesenchymal stem cells derived from bone marrow
|Abdel Aziz MT,Wassef MA,Ahmed HH,Rashed L,Mahfouz S,Aly MI,Hussein RE,Abdelaziz M||Diabetology & metabolic syndrome (6:null)||2014|
Overexpression of MMP-1 and VEGF-C is associated with a less favorable prognosis in esophageal squamous cell carcinoma.
MA5-14573 was used in immunohistochemistry to study the prognostic significance of the overexpression of MMP-1 and VEGF-C in patients with esophageal squamous cell carcinoma
|Tao YS,Ma XY,Chai DM,Ma L,Feng ZZ,Cheng ZN,Lai MD||Onkologie (35:651)||2012|
Giant cell lesions of the jaws: does the level of vascularity and angiogenesis correlate with behavior?
MA5-14573 was used in immunohistochemistry to study the correlation between vascularity and angiogenesesis and the agressiveness of giant cell lesions of the jaw
|Peacock ZS,Jordan RC,Schmidt BL||Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (70:1860)||2012|
Anti-human vascular endothelial growth factor (VEGF) antibody selection for immunohistochemical staining of proliferating blood vessels.
MA5-14573 was used in immunohistochemistry to evaluate suitable vascular endothelial growth factor antibodies for the detection of proliferating blood vessels
|van der Loos CM,Meijer-Jorna LB,Broekmans ME,Ploegmakers HP,Teeling P,de Boer OJ,van der Wal AC||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (58:109)||2010|
Local chronic hypoperfusion secondary to sinus high pressure seems to be mainly responsible for the formation of intracranial dural arteriovenous fistula.
MA5-14573 was used in immunohistochemistry to investigate the role of chronic local hypoperfusion in the formation of intracranial dural arteriovenous fistula
|Chen L,Mao Y,Zhou LF||Neurosurgery (64:973)||2009|
Overexpression of phosphorylated mammalian target of rapamycin predicts lymph node metastasis and prognosis of chinese patients with gastric cancer.
MA5-14573 was used in immunohistochemistry to examine the prognostic significance of mammalian target of rapamycin and its phoshorylated form in gastric cancer
|Yu G,Wang J,Chen Y,Wang X,Pan J,Li G,Jia Z,Li Q,Yao JC,Xie K||Clinical cancer research : an official journal of the American Association for Cancer Research (15:1821)||2009|
Expression of hypoxia-inducible factor-1 and vascular endothelial growth factor in response to venous hypertension.
MA5-14573 was used in immunohistochemistry to investigate the influence of venous hypertension on the expression of hypoxia-inducible factor-1 and vascular endothelial growth factor
|Zhu Y,Lawton MT,Du R,Shwe Y,Chen Y,Shen F,Young WL,Yang GY||Neurosurgery (59:687)||2006|
Organic dust-induced activation, adhesion to substrate and expression of intercellular adhesion molecules in THP-1 monocytes.
MA5-14573 was used in immunocytochemistry to investigate the activation, adhesion, and expression of intercellular adhesion molecules induced by organic dust in THP-1 monocytes
|Burvall K,Palmberg L,Larsson K||Life sciences (80:1598)||2007|
vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor
MVCD1; VEGF; VEGFA; VPF