Invitrogen

VEGF Recombinant Rabbit Monoclonal Antibody (1H12L17)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (92) VEGF antibodies

Datasheet

Protocols

Datasheet

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VEGF Antibody (701124) in WB

Western blot analysis of active recombinant mouse VEGF using a VEGF recombinant rabbit monoclonal antibody (Product # 701124) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~40kDa.

Product Image Gallery

VEGF Antibody — Fig. 3 IFN-alpha2a-NGR decreased the expression of VEGF and bFGF. Paraffin-embedded tumor tissues from A549 bearing-mice treated with IFN-alpha2a-NGR or IFN-alpha2a were obtained from antitumor studies, prepared and coated on polylysine-coated slides. The sections were stained with the mouse anti-human- bFGF (Santa Cruz), VEGF (Zymed) antibodies to examine VEGF ( a ) and bFGF ( b ) expression in tumor tissues. ( c ) Western blot analysis of VEGF and bFGF expression in tumor tissues. ( d ) The Real-time PCR assay of VEGF and bFGF mRNA level in tumor tissues. For all experiments, each group was performed in triplicate or quadruplicate; the mean value +- SD of a representative of three independent assays is shown. Data were analyzed by one-way ANOVA statistical tests. * p < 0.05 {TM}

Product Details

701124

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Product Specifications

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

1H12L17

Immunogen

Recombinant protein corresponding to amino acids 206-317 of mouse vascular endothelial growth factor A

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532401

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.^M
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Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.^M
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Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recombinant IFN-α2a-NGR exhibits higher inhibitory function on tumor neovessels formation compared with IFN-α2a in vivo and in vitro.

Cytotechnology

Li W,Hao Q,He L,Meng J,Li M,Xue X,Zhang C,Li H,Zhang W,Zhang Y

Published figure using VEGF monoclonal antibody (Product # 701124) in Western Blot

Tue Dec 01 00:00:00 UTC 2015

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