|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 206–317 of mouse vascular endothelial growth factor A|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
VEGF belongs to a family of heparin binding growth factors and is the major mediator of vasculogenesis and angiogenesis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. VEGF is a 45 kDa homodimeric glycoprotein, but other isoforms can be produced through alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Recombinant IFN-¿2a-NGR exhibits higher inhibitory function on tumor neovessels formation compared with IFN-¿2a in vivo and in vitro.
701124 was used in western blot to evaluate the mechanisms of NGR-fused IFN-alpha2a on angiogenesis and tumor formation
|Li W,Hao Q,He L,Meng J,Li M,Xue X,Zhang C,Li H,Zhang W,Zhang Y||Cytotechnology (67:1039)||2015|