Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is placenta tissue.
FLK-1/KDR/VEGFR2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to FLT-1 and FLT-4.
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Protein Aliases: 6130401C07; CD309; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; Flk1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); kinase NYK; Krd-1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble vascular endothelial growth factor receptor 2; soluble VEGFR2; sVEGFR-2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF receptor-2; VEGFR-2; VEGFR2
Gene Aliases: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2
UniProt ID: (Human) P35968