|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant extracellular domain of mouse VEGF receptor 2, expressed in mouse NSO cells.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Immunohistochemistry (IHC)||5-15 µg/ml|
|Neutralization (Neu)||5-50 ng/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20207 detects VEGF Receptor 2 in mouse samples.
PA1-20207 has been successfully used in ELISA, neutralization, immunohistochemistry and Western blot procedures. Neutralization: Anti-Mouse VEGF Receptor 2 has the ability to neutralize the biological activity of recombinant mouse VEGF Receptor 2 on HUVE (human umbilical vein endothelial) cells in the presence of recombinant mouse VEGF Receptor 2/Fc (50 ng/ml) and recombinant mouse VEGF (5 ng/ml).
The PA1-20207 immunogen is recombinant extracellular domain of mouse VEGF receptor 2, expressed in mouse NSO cells.
Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF Receptor 1 and VEGF Receptor 2 are both expressed in an endothelial cell-specific manner. They are detectable in virtually all tissues in adults and embryos. Monocytes express VEGF Receptor 1 and VEGF Receptor 2. VEGF Receptor 2 is involved in commitment of endothelial-cell lineages and to cell proliferation, while VEGF Receptor 1 seems to be responsible for guiding endothelial cells into the proper spatial organization of the lumen-containing vessels. VEGF Receptor 2 binds VEGF with high affinity but does not bind PIGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.