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Invitrogen
VMA2 Monoclonal Antibody (13D11B2)
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Product Details
A-6427
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Published species
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
13D11B2
Immunogen
Purified S. cerevisiae vacuole membranes
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This clone recognizes the 60 kDa subunit 1 (B-subunit, the product of the VMA2 or VAT2 gene) of S. cerevisiae. By Western blot, this can be used to identify organelles during subcellular fractionation of yeast membranes.
Target Information
Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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