Product References
The ERAD system is restricted by elevated ceramides.
Science advances
Hwang J,Peterson BG,Knupp J,Baldridge RD
A-6427 was used in Western Blot to reveal a previously unrecognized mechanism in which ER membrane lipid remodeling changes the activity of ERAD.
Fri Jan 13 00:00:00 UTC 2023
Changes in transcription start sites of Zap1-regulated genes during zinc deficiency: Implications for HNT1 gene regulation.
Molecular microbiology
Tatip S,Taggart J,Wang Y,MacDiarmid CW,Eide DJ
A-6427 was used in Western Blotting to explored how alterations in transcription start sites affected expression of genes regulated by the Zap1 transcriptional activator of Saccharomyces cerevisiae.
Wed Jan 01 00:00:00 UTC 2020
Mitochondrial Localization of the Yeast Forkhead Factor Hcm1.
International journal of molecular sciences
Rodríguez Colman MJ,Ros J,Cabiscol E
A-6427 was used in Western Blotting to show a new localization of Hcm1 and suggest that it acts as a mitochondrial transcription factor regulating the metabolism of this organelle.
Wed Dec 16 00:00:00 UTC 2020
Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1.
The Biochemical journal
Szatkowska R,Garcia-Albornoz M,Roszkowska K,Holman SW,Furmanek E,Hubbard SJ,Beynon RJ,Adamczyk M
A-6427 was used in Western Blotting to analyse the effects of altered RNA polymerase III activity on the Saccharomyces cerevisiae proteome and metabolism under glucose-rich conditions.
Thu Apr 04 00:00:00 UTC 2019
Novel c.G630A TCIRG1 mutation causes aberrant splicing resulting in an unusually mild form of autosomal recessive osteopetrosis.
Journal of cellular biochemistry
Zirngibl RA,Wang A,Yao Y,Manolson MF,Krueger J,Dupuis L,Mendoza-Londono R,Voronov I
A-6427 was used in Western Blotting to show that the ΔE56 truncated protein is not functional, suggesting that the mild ARO phenotype observed in the patient is likely due to the residual full-length protein expression.
Tue Oct 01 00:00:00 UTC 2019
Architecture of Lipid Droplets in Endoplasmic Reticulum Is Determined by Phospholipid Intrinsic Curvature.
Current biology : CB
Choudhary V,Golani G,Joshi AS,Cottier S,Schneiter R,Prinz WA,Kozlov MM
A-6427 was used in Western Blotting to study the mechanism by which cells regulate lipid droplet integration into the endoplasmic reticulum.
Mon Mar 19 00:00:00 UTC 2018
Low RNA Polymerase III activity results in up regulation of HXT2 glucose transporter independently of glucose signaling and despite changing environment.
PloS one
Adamczyk M,Szatkowska R
Published figure using V-ATPase 60 kDa subunit monoclonal antibody (Product # A-6427) in Western Blot
Fri Nov 03 00:00:00 UTC 2017
The Heme Transport Capacity of LHR1 Determines the Extent of Virulence in Leishmania amazonensis.
PLoS neglected tropical diseases
Renberg RL,Yuan X,Samuel TK,Miguel DC,Hamza I,Andrews NW,Flannery AR
A-6427 was used in western blot to study Leishmania LHR1 and heme uptake in Saccharomyces cerevisiae
Fri May 01 00:00:00 UTC 2015
Reducing sphingolipid synthesis orchestrates global changes to extend yeast lifespan.
Aging cell
Liu J,Huang X,Withers BR,Blalock E,Liu K,Dickson RC
Published figure using V-ATPase 60 kDa subunit monoclonal antibody (Product # A-6427) in Western Blot
Tue Oct 01 00:00:00 UTC 2013
Reducing signs of aging and increasing lifespan by drug synergy.
Aging cell
Huang X,Liu J,Withers BR,Samide AJ,Leggas M,Dickson RC
Published figure using V-ATPase 60 kDa subunit monoclonal antibody (Product # A-6427) in Western Blot
Thu Aug 01 00:00:00 UTC 2013
Topology, glycosylation and conformational changes in the membrane domain of the vacuolar H+-ATPase a subunit.
Journal of cellular biochemistry
Kartner N,Yao Y,Bhargava A,Manolson MF
A-6427 was used in western blot to construct and experimentally test a predictive model of the membrane insertion of the yeast a subunit, Vph1p.
Mon Jul 01 00:00:00 UTC 2013
Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2.
FEBS letters
Rodríguez-Peña JM,Díez-Muñiz S,Bermejo C,Nombela C,Arroyo J
A-6427 was used in western blot to elucidate the signaling pathways involved in maintaining cell wall integrity.
Fri Nov 15 00:00:00 UTC 2013
Structural analysis of the N-terminal domain of subunit a of the yeast vacuolar ATPase (V-ATPase) using accessibility of single cysteine substitutions to chemical modification.
The Journal of biological chemistry
Liberman R,Cotter K,Baleja JD,Forgac M
A-6427 was used in immunoprecipitation to model the N-terminal domain of Vph1p and test this model.
Fri Aug 02 00:00:00 UTC 2013
BTN1, the Saccharomyces cerevisiae homolog to the human Batten disease gene, is involved in phospholipid distribution.
Disease models & mechanisms
Padilla-López S,Langager D,Chan CH,Pearce DA
A-6427 was used in western blot to test if cells lacking BTN1 have altered membrane phospholipid content.
Thu Mar 01 00:00:00 UTC 2012
pH-dependent localization of Btn1p in the yeast model for Batten disease.
Disease models & mechanisms
Wolfe DM,Padilla-Lopez S,Vitiello SP,Pearce DA
A-6427 was used in western blot to report how extracellular pH alterations affect Btn1p
Sat Jan 01 00:00:00 UTC 2011
The reconstructed ancestral subunit a functions as both V-ATPase isoforms Vph1p and Stv1p in Saccharomyces cerevisiae.
Molecular biology of the cell
Finnigan GC,Hanson-Smith V,Houser BD,Park HJ,Stevens TH
Published figure using V-ATPase 60 kDa subunit monoclonal antibody (Product # A-6427) in Western Blot
Thu Sep 01 00:00:00 UTC 2011
A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae.
Molecular & cellular proteomics : MCP
Dokudovskaya S,Waharte F,Schlessinger A,Pieper U,Devos DP,Cristea IM,Williams R,Salamero J,Chait BT,Sali A,Field MC,Rout MP,Dargemont C
A-6427 was used in western blot to identify the SEA complex is a member of a family of membrane coating and vesicle tethering assemblies needed for intracellular trafficking, amino acid biogenesis, and response to nitrogen starvation.
Wed Jun 01 00:00:00 UTC 2011
Definition of membrane topology and identification of residues important for transport in subunit a of the vacuolar ATPase.
The Journal of biological chemistry
Toei M,Toei S,Forgac M
A-6427 was used in western blot to describe the hemichannel of H(+)-ATPases.
Fri Oct 07 00:00:00 UTC 2011
The tether connecting cytosolic (N terminus) and membrane (C terminus) domains of yeast V-ATPase subunit a (Vph1) is required for assembly of V0 subunit d.
The Journal of biological chemistry
Ediger B,Melman SD,Pappas DL,Finch M,Applen J,Parra KJ
A-6427 was used in western blot to identify important functional residues of subunit a of V-ATPases
Fri Jul 17 00:00:00 UTC 2009
Biochemical and functional characterization of the actin-binding activity of the B subunit of yeast vacuolar H+-ATPase.
The Journal of experimental biology
Zuo J,Vergara S,Kohno S,Holliday LS
A-6427 was used in immunoprecipitation to investigate the actin-binding activity of the B subunit of yeast V-ATPase
Tue Apr 01 00:00:00 UTC 2008
Molecular identification, immunolocalization, and functional activity of a vacuolar-type H(+)-ATPase in bovine rumen epithelium.
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
Albrecht E,Kolisek M,Viergutz T,Zitnan R,Schweigel M
A-6427 was used in western blot to assess the expression, localization, and functionality of vacuolar-type H(+)-ATPase and Na(+)/K(+)-ATPase in the bovine rumen epithelium
Sat Mar 01 00:00:00 UTC 2008
Defective assembly of a hybrid vacuolar H(+)-ATPase containing the mouse testis-specific E1 isoform and yeast subunits.
Biochimica et biophysica acta
Hayashi K,Sun-Wada GH,Wada Y,Nakanishi-Matsui M,Futai M
A-6427 was used in immunocytochemistry to characterize the properties of E1/yeast hybrid V-ATPase
Wed Oct 01 00:00:00 UTC 2008
Functional characterization of the N-terminal domain of subunit H (Vma13p) of the yeast vacuolar ATPase.
The Journal of biological chemistry
Flannery AR,Stevens TH
A-6427 was used in western blot to describe the properties of V-ATPase complexes
Fri Oct 24 00:00:00 UTC 2008
Retrophosphorylation of Mkk1 and Mkk2 MAPKKs by the Slt2 MAPK in the yeast cell integrity pathway.
The Journal of biological chemistry
Jiménez-Sánchez M,Cid VJ,Molina M
A-6427 was used in western blot to report that cell wall integrity microtubule-associated protein kinase kinases are targets for their downstream MAPK
Fri Oct 26 00:00:00 UTC 2007
A vH+-ATPase is present in cultured sheep ruminal epithelial cells.
American journal of physiology. Gastrointestinal and liver physiology
Etschmann B,Heipertz KS,von der Schulenburg A,Schweigel M
A-6427 was used in western blot to identify and characterize a vacuolar-type H+-ATPase in sheep ruminal epithelial cells
Fri Dec 01 00:00:00 UTC 2006
Effects of human a3 and a4 mutations that result in osteopetrosis and distal renal tubular acidosis on yeast V-ATPase expression and activity.
The Journal of biological chemistry
Ochotny N,Van Vliet A,Chan N,Yao Y,Morel M,Kartner N,von Schroeder HP,Heersche JN,Manolson MF
A-6427 was used in western blot to study the result in osteopetrosis and distal renal tubular acidosis on yeast V-ATPase activity and expression due to effects of human a3 and a4 mutations
Fri Sep 08 00:00:00 UTC 2006
Involvement of the nonhomologous region of subunit A of the yeast V-ATPase in coupling and in vivo dissociation.
The Journal of biological chemistry
Shao E,Forgac M
A-6427 was used in western blot to propose that the dependence of the dissociation of the V-ATPase on catalytic activity is due to neutralization of the yeast vacuole
Fri Nov 19 00:00:00 UTC 2004
The glycolytic enzyme aldolase mediates assembly, expression, and activity of vacuolar H+-ATPase.
The Journal of biological chemistry
Lu M,Sautin YY,Holliday LS,Gluck SL
A-6427 was used in immunoprecipitation and western blot to investigate how ATP is supplied for V-ATPase-mediated hydrolysis and for coupling of proton transport.
Fri Mar 05 00:00:00 UTC 2004
Identification of an organelle-specific myosin V receptor.
The Journal of cell biology
Ishikawa K,Catlett NL,Novak JL,Tang F,Nau JJ,Weisman LS
A-6427 was used in immunocytochemistry to study and characterize organelle-specific myosin V receptor
Mon Mar 17 00:00:00 UTC 2003
The amino-terminal domain of the E subunit of vacuolar H(+)-ATPase (V-ATPase) interacts with the H subunit and is required for V-ATPase function.
The Journal of biological chemistry
Lu M,Vergara S,Zhang L,Holliday LS,Aris J,Gluck SL
A-6427 was used in immunoprecipitation to identify proteins that bind E subunit of V-ATPase
Fri Oct 11 00:00:00 UTC 2002
Quality control in the yeast secretory pathway: a misfolded PMA1 H+-ATPase reveals two checkpoints.
The Journal of biological chemistry
Ferreira T,Mason AB,Pypaert M,Allen KE,Slayman CW
A-6427 was used in immunocytochemistry to develop a new probe to study the yeast secretory system.
Fri Jun 07 00:00:00 UTC 2002
Arg-735 of the 100-kDa subunit a of the yeast V-ATPase is essential for proton translocation.
Proceedings of the National Academy of Sciences of the United States of America
Kawasaki-Nishi S,Nishi T,Forgac M
A-6427 was used in western blot to identify the residues required for proton transport by the V-ATPases.
Tue Oct 23 00:00:00 UTC 2001
Interaction of the endoplasmic reticulum alpha 1,2-mannosidase Mns1p with Rer1p using the split-ubiquitin system.
Journal of cell science
Massaad MJ,Herscovics A
A-6427 was used in immunocytochemistry to determine the results of Mns1p and Rer1p interactions.
Sat Dec 01 00:00:00 UTC 2001
A novel mechanism for localizing membrane proteins to yeast trans-Golgi network requires function of synaptojanin-like protein.
Molecular biology of the cell
Ha SA,Bunch JT,Hama H,DeWald DB,Nothwehr SF
A-6427 was used in immunocytochemistry to screen for genes required for the slow delivery protein pathway using yeast.
Mon Oct 01 00:00:00 UTC 2001
The amino-terminal domain of the vacuolar proton-translocating ATPase a subunit controls targeting and in vivo dissociation, and the carboxyl-terminal domain affects coupling of proton transport and ATP hydrolysis.
The Journal of biological chemistry
Kawasaki-Nishi S,Bowers K,Nishi T,Forgac M,Stevens TH
A-6427 was used in western blot to examine the role of "a" subunit of the vacuolar proton-translocating ATPase in cellular localization of the complex.
Fri Dec 14 00:00:00 UTC 2001
Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p.
The Journal of cell biology
Nothwehr SF,Ha SA,Bruinsma P
A-6427 was used in immunocytochemistry to study the role of Vps35p in transporting cargo proteins.
Mon Oct 16 00:00:00 UTC 2000
Regulation and reversibility of vacuolar H(+)-ATPase.
The Journal of biological chemistry
Hirata T,Nakamura N,Omote H,Wada Y,Futai M
A-6427 was used in immunocytochemistry to examine the regulation and reversibility of V-ATPase
Fri Jan 07 00:00:00 UTC 2000
Cysteine scanning mutagenesis of the noncatalytic nucleotide binding site of the yeast V-ATPase.
The Journal of biological chemistry
Vasilyeva E,Liu Q,MacLeod KJ,Baleja JD,Forgac M
A-6427 was used in western blot to identify residues involved in the formation of the noncatalytic nucleotide binding sites of the vacuolar proton-translocating adenosine triphosphatase.
Fri Jan 07 00:00:00 UTC 2000
Distinct domains within Vps35p mediate the retrieval of two different cargo proteins from the yeast prevacuolar/endosomal compartment.
Molecular biology of the cell
Nothwehr SF,Bruinsma P,Strawn LA
Published figure using V-ATPase 60 kDa subunit monoclonal antibody (Product # A-6427) in Immunocytochemistry
Thu Apr 01 00:00:00 UTC 1999
The yeast GRD20 gene is required for protein sorting in the trans-Golgi network/endosomal system and for polarization of the actin cytoskeleton.
Molecular biology of the cell
Spelbrink RG,Nothwehr SF
A-6427 was used in immunocytochemistry to identify GRD20 as a gene that is important for protein sorting in the TGN/endosomal system of Saccharomyces cerevisiae.
Wed Dec 01 00:00:00 UTC 1999
Substrate- and inhibitor-induced conformational changes in the yeast V-ATPase provide evidence for communication between the catalytic and proton-translocating sectors.
The Journal of biological chemistry
Landolt-Marticorena C,Kahr WH,Zawarinski P,Correa J,Manolson MF
A-6427 was used in western blot to study nucleotide- and cation- induced changes in the catalytic core of the V-ATPase
Fri Sep 10 00:00:00 UTC 1999
The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane.
The Journal of biological chemistry
Urbanowski JL,Piper RC
A-6427 was used in immunocytochemistry to elucidate how the vacuole contributes to iron metabolism.
Fri Dec 31 00:00:00 UTC 1999
Helical interactions and membrane disposition of the 16-kDa proteolipid subunit of the vacuolar H(+)-ATPase analyzed by cysteine replacement mutagenesis.
The Journal of biological chemistry
Harrison MA,Murray J,Powell B,Kim YI,Finbow ME,Findlay JB
A-6427 was used in western blot to elucidate proton translocation by the vacuolar H(+)-ATPase
Fri Sep 03 00:00:00 UTC 1999
VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly.
The Journal of biological chemistry
Jackson DD,Stevens TH
A-6427 was used in western blot to characterize Vma12p
Fri Oct 10 00:00:00 UTC 1997
Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant.
Molecular biology of the cell
Rieder SE,Banta LM,Köhrer K,McCaffery JM,Emr SD
A-6427 was used in immunocytochemistry to suggest that Vps28p facilitates the formation of transport intermediates required for efficient transport out of the prevacuolar endosome
Sat Jun 01 00:00:00 UTC 1996
VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae.
The Journal of cell biology
Piper RC,Cooper AA,Yang H,Stevens TH
A-6427 was used in immunocytochemistry to suggest that Vps27p controls membrane traffic through the prevacuolar/endosomal compartment
Wed Nov 01 00:00:00 UTC 1995
The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization.
Molecular biology of the cell
Cereghino JL,Marcusson EG,Emr SD
A-6427 was used in immunocytochemistry to determine the structural features and intercompartmental transport of carboxypeptidase Y
Fri Sep 01 00:00:00 UTC 1995
Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.
Molecular biology of the cell
Raymond CK,Howald-Stevenson I,Vater CA,Stevens TH
A-6427 was used in immunocytochemistry to analyze sorting defects in vacuolar protein sorting mutants in Saccharomyces cerevisiae
Tue Dec 01 00:00:00 UTC 1992
