|Immunocytochemistry (ICC)||1-3 ug/ml|
|Immunofluorescence (IF)||1-3 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||2-5 ug/ml|
|Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 502–515 of human VPS34|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
VPS34 is a class III phosphatidylinositol 3-kinase (PI3K). PI3K are lipid kinases that regulate vital cellular processes, including signal transduction, membrane trafficking, cytoskeletal regulation and apoptosis. VPS34 plays a role in insulin-stimulated mitogenesis. It is required for internal vesicle formation within multivesicular endosomes. VPS34 and its adapter protein p150 are Rab7 interacting partners, and the hVPS34/p150 complex co-localizes with Rab7 on late endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hVps34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3
Gene Aliases: 5330434F23Rik; hVps34; PIK3C3; VPS34
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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