Immunofluorescent analysis of VR1 using a VR1 polyclonal antibody (Product # PA1-29770) showing expression patterns of TLR4 and CD14 in trigeminal sensory neurons. White arrows depict examples of neurons expressing both markers for each row of three images, and yellow arrows depict examples of neurons that express one but not both markers. Human trigeminal neurons were evaluated for co-localization of TLR4 (Panels A,D), CD14 (Panels B,J), with a marker for the capsaicinsensitive subclass of nociceptors (TRPV1, Panels B,C for TLR4 and Panels K,L for CD14), or a marker of myelinated sensory neurons (N52, Panels E,F). Rat trigeminal neurons were evaluated for co-localization of TLR4 with TRPV1 (Panels G-I) and CD14 with TRPV1 (Panels M-O). The addition of blocking peptide or the deletion of primary or secondary antisera produced a complete loss of signal in both the human and rat tissues. Scale bar is 100 um for Panels A-F and J-L, and 200 um for Panels G-I and M-O.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Guinea pig / IgG|
|Immunogen||Peptide corresponding to residues YTGSLKPEDAEVFKDSMVPGEK of the carboxy-terminus of rat VR1|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29770 detects VR1 from human and rat samples.
PA1-29770 has been successfully used in immunohistochemistry applications.
The PA1-29770 immunogen is YTGSLKPEDAEVFKDSMVPGEK Corresponding to residues of the carboxy-terminus of rat VR1.
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.