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Western blot analysis of VR1 in HEK293 cells using VR1 polyclonal antibody (Product # PA1-30911). (C) TRPV1 in HEK293 cells was not tyrosine phosphorylated. HEK293 cells expressing combinations of TRPV1, p75, and trkA, as indicated in the figure, were treated with either NGF or vehicle and then immunoprecipitated with anti-trkA (lanes 1-7) or anti-TRPV1 (lanes 8-13) antibodies. Lanes 1-7 were separated from lanes 8-13 and the membranes were probed with anti-trkA antibody and anti-TRPV1 antibody, respectively (top). Segments of the membrane were then reapposed for imaging. The membranes were then stripped and reprobed with anti-phosphotyrosine antibody (bottom).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RASLDSEESESPPQENSC Corresponding to residues 4-21 of the amino-terminus of rat VR1|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30911 detects VR1 from rat samples.
PA1-30911 has been successfully used in immunocytochemistry, immunohistochemistry, Western blot applications.
The PA1-30911 immunogen is: RASLDSEESESPPQENSC Corresponding to residues 4-21 of the amino-terminus of rat VR1
VR1 (vanilloid receptor 1) is a channel activated by capsaicin, the pungent ingredient in hot peppers. It was isolated from sensory neurons. Sequence analysis suggests that VR1 has six transmembrane domains and intracellular N- and C-termini. It shares structural similarities with the family of putative store-operated calcium channels. VR1 is expressed by nociceptive sensory neurons. It is activated by noxious heat and is thought to function as transducer of noxious thermal stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Transient receptor potential cation channel subfamily V member 1; transient receptor potential vanilloid 1 supraoptic nucleus; TrpV1; Vanilloid receptor 1; vanilloid receptor subtype 1; vanilloid receptor type 1 like protein 1; Vanilloid receptor type 1-like; vanilloid type 1 receptor; VR.5'sv; Vr1; Vr1l1
Trpv1; TRPV1_SON; VR.5'sv; Vr1; Vr1l; Vr1l1