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Immunofluorescent analysis of VRK1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a VRK1 monoclonal antibody (Product # MA1-828) at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fusion protein GST-VRK1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:100 - 1:500|
|Immunohistochemistry (IHC)||1:100 - 1:500|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-828 detects VRK1 from human and mouse samples.
MA1-828 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry applications. For Western blotting, it is recommended to block with 3% BSA (not milk).
The MA1-828 immunogen is recombinant fusion protein GST-VRK1.
Human vaccinia-related kinase 1 (VRK1) is a new kinase related to a poxvirus kinase, and more distantly to the casein kinase 1 family. VRK1 shows strong autophosphorylating activity in several Ser and Thr residues. VRK has also been found to be an upstream regulator of p53 that belongs to a new signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MGC117401; MGC138280; Serine/threonine-protein kinase 51PK; Serine/threonine-protein kinase VRK1; Vaccinia related kinase 1; vaccinia virus B1R-related kinase 1; Vaccinia-related kinase 1
51PK; PCH1; PCH1A; VRK1