|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||An 18 amino acid peptide near the center of human VTCN1|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 - 2 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is EL4 cell lysate.
PA5-34501 can be used with blocking peptide PEP-1544.
T cell immunity plays a critical role in host immune surveillance of tumour cell growth and metastatic spread. VTCN1, also known as B7-H4, is a member of the B7 family of immune regulatory molecules. Expressed primarily on the membrane of lymphoid cells, VTCN1 is an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of VTCN1 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumour cells suppress T cell immunity and facilitate tumour progression. VTCN1 may thus be a useful biomarker for the early detection of ovarian cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.