Frozen human skin tissue sections were fixed with paraformaldehyde and stained with PA1-748 (1:250) followed by a biotin-conjugated donkey anti-rabbit IgG secondary antibody and streptavidin-HRP/tyrimide-fluorescent detection system. Vanilloid Receptor 1 staining is shown in red. Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues T(7) D L G A A A D P L Q K D T C(21) of human VR1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-748 detects vanilloid receptor 1 (VR1) from human bladder and skin.
PA1-748 has been successfully used in immunofluorescence and immunohistochemistry procedures. Immunohistochemical staining of VR1 in human glabrous skin using this antibody results in the staining of epidermal and dermal cells, as well as free nerve fibers and Merkel cells. Immunofluorescent staining of VR1 in human prostatic urethra using PA1-748 results in fibrous staining within the epithelial cells.
The PA1-748 immunogen is a synthetic peptide corresponding to residues T(7) D L G A A A D P L Q K D T C(21) of human VR1. PA1-748 immunizing peptide (Cat. # PEP-202) is available for use in neutralization and control experiments.
Vanilloid receptor 1 (VR1), also known as capsaicin receptor, responds to both capsaicin, the pungent ingredient in hot peppers, and thermal stimuli within the noxious temperature range. This receptor is structurally related to the TRP family of ion channels and is predicted to contain 6 trans-membrane domains, a P-loop membrane domain and 3 ankyrin repeat domains. VR1 is highly permeable to divalent cations, including calcium and magnesium, as well as the monovalent cations sodium and potassium. Expression of VR1 appears to be limited to neurons within the sensory ganglia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activation of endogenous TRPV1 fails to induce overstimulation-based cytotoxicity in breast and prostate cancer cells but not in pain-sensing neurons.
PA1-748 was used in western blot to assess the failure to induce overstimulation-based cytotoxicity in breast and prostate cancer cells and not pain-sensing neurons after activation of endogenous TRPV1
|Pecze L,Jósvay K,Blum W,Petrovics G,Vizler C,Oláh Z,Schwaller B||Biochimica et biophysica acta (1863:2054)||2016|
Human keratinocytes are vanilloid resistant.
PA1-748 was used in western blot to study the mechanism of the vanilloid resistance in human keratinocytes
|Pecze L,Szabó K,Széll M,Jósvay K,Kaszás K,Kúsz E,Letoha T,Prorok J,Koncz I,Tóth A,Kemény L,Vizler C,Oláh Z||PloS one (3:null)||2008|
The neurochemical changes in the innervation of human colonic mesenteric and submucosal blood vessels in ulcerative colitis and Crohn's disease.
PA1-748 was used in immunohistochemistry to study colonic mesenteric and submucosal blood vessel ennervation in patients with ulcerative colitis and Crohn's disease
|de Fontgalland D,Brookes SJ,Gibbins I,Sia TC,Wattchow DA||Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (26:731)||2014|
TRPV1 and TRPA1 stimulation induces MUC5B secretion in the human nasal airway in vivo.
PA1-748 was used in immunohistochemistry to study the effect of TRPV1 and TRPA1 on MUC5B secretion in the human nasal airway in vivo
|Alenmyr L,Herrmann A,Högestätt ED,Greiff L,Zygmunt PM||Clinical physiology and functional imaging (31:435)||2011|
Transient receptor potential vanilloid 1, vanilloid 2 and melastatin 8 immunoreactive nerve fibers in human skin from individuals with and without Norrbottnian congenital insensitivity to pain.
PA1-748 was used in immunohistochemistry to study the role of transient receptor potential vanilloid 1, vanilloid 2 and melastatin 8 in nociceptive primary sensory neurons in human skin
|Axelsson HE,Minde JK,Sonesson A,Toolanen G,Högestätt ED,Zygmunt PM||Neuroscience (162:1322)||2009|
Deletion of vanilloid receptor 1-expressing primary afferent neurons for pain control.
PA1-748 was used in immunohistochemistry to study the effect of nociceptive neuronal or nerve terminal deletion on experimental inflammatory hyperalgesia and neurogenic inflammation.
|Karai L,Brown DC,Mannes AJ,Connelly ST,Brown J,Gandal M,Wellisch OM,Neubert JK,Olah Z,Iadarola MJ||The Journal of clinical investigation (113:1344)||2004|