|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide corresponding to residues A(27) P M A E G G G Q N H H E V V K F M D(45) of human VEGF.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OPA1-10110 detects recombinant human VEGF.
OPA1-10110 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~21 kDa protein representing recombinant human VEGF.
OPA1-10110 immunogen is a synthetic peptide corresponding to residues A(27) P M A E G G G Q N H H E V V K F M D(45) of human VEGF.
The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.