|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||0.5-2 µg/ml|
|Tested Species reactivity||Bovine, Human, Sheep, Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human VEGF189 expressed in E.coli.|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each.
For IHC using paraffin sections, microwave treatment with Tris HCl, pH 10 buffer (NOT citrate buffer) is necessary.
Suggested positive control: antigen standard for VEGFA (transient overexpression lysate), recombinant human VEGF protein.
The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: angiogenic factor; Vascular Endothelial Growth Factor; vascular endothelial growth factor 164; Vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular Permeability Factor; VEGF-A; VEGFA; VPF
Gene Aliases: BOS_21465; eVEGF120; eVEGF164; MVCD1; VEGF; VEGF-A; VEGFA; VPF
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.