|Tested species reactivity||Human|
|Published species reactivity||Rabbit, Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C G G D(436) P E A A E(441) of human Versican V0/V1 Neo.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1748A detects versican from human samples.
PA1-1748A has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~70 kDa protein representing versican from mouse cumulus oocyte complexes.
PA1-1748A immunogen is a synthetic peptide corresponding to residues C G G D(436) P E A A E(441) of human Versican V0/V1 Neo. This sequence is completely conserved between mouse and rat. This immunizing peptide (PEP-235) can be used for neutralization and control experiments. This polyclonal antibody has been referred to as: JSCDPE or DPEAAE.
Versican is a member of the family of large aggregating proteoglycans (also including aggrecan, brevican, and neurocan). The protein shows wide tissue distribution which includes fibrous, articular, and elastic cartilages, as well as nervous, epidermal, arterial, and loose connective tissues. Versican V1 has been shown to enhance cell proliferation, and also induce cell transformation and protect cells from apoptosis. This was demonstrated through its ability to downregulate the expression of proapoptotic Bad. Versican's existence as a extracellular matrix protein in human airways has also lead to the discovery of an inflammatory role in asthmatics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signalling.
PA1-1748A was used in immunohistochemistry - paraffin section to characterize the epithelial gain of MEKK3-KLF2/4 signaling due to cerebral cavernous malformations
|Zhou Z,Tang AT,Wong WY,Bamezai S,Goddard LM,Shenkar R,Zhou S,Yang J,Wright AC,Foley M,Arthur JS,Whitehead KJ,Awad IA,Li DY,Zheng X,Kahn ML||Nature (532:122)||2016|
Galnt1 is required for normal heart valve development and cardiac function.
PA1-1748A was used in immunohistochemistry - paraffin section to study heart valve development and cardiac function in Galnt1 KO mice
|Tian E,Stevens SR,Guan Y,Springer DA,Anderson SA,Starost MF,Patel V,Ten Hagen KG,Tabak LA||PloS one (10:null)||2015|
Correlation of Versican Expression, Accumulation, and Degradation during Embryonic Development by Quantitative Immunohistochemistry.
PA1-1748A was used in immunohistochemistry to determine versican expression in the murine lung and central nervous system
|Snyder JM,Washington IM,Birkland T,Chang MY,Frevert CW||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (63:952)||2015|
Versican localizes to the nucleus in proliferating mesenchymal cells.
PA1-1748A was used in immunocytochemistry and western blot to discuss intracellular functions of versican
|Carthy JM,Abraham T,Meredith AJ,Boroomand S,McManus BM||Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology (24:368)||2015|
Elevated expression of long intergenic non-coding RNA HOTAIR in a basal-like variant of MCF-7 breast cancer cells.
PA1-1748A was used in western blot to assess the function of HOTAIR in breast cancer cells that are resistant to TNF-alpha-induced cell death
|Zhuang Y,Nguyen HT,Burow ME,Zhuo Y,El-Dahr SS,Yao X,Cao S,Flemington EK,Nephew KP,Fang F,Collins-Burow B,Rhodes LV,Yu Q,Jayawickramarajah J,Shan B||Molecular carcinogenesis (54:1656)||2015|
Differential effects of caveolin-1 and -2 knockdown on aqueous outflow and altered extracellular matrix turnover in caveolin-silenced trabecular meshwork cells.
PA1-1748A was used in immunohistochemistry and western blot to investigate the role of caveolin-1 and -2 in aqueous outflow and extracellular matrix turnover in trabecular meshwork cells
|Aga M,Bradley JM,Wanchu R,Yang YF,Acott TS,Keller KE||Investigative ophthalmology and visual science (55:5497)||2014|
Biosynthesis and expression of a disintegrin-like and metalloproteinase domain with thrombospondin-1 repeats-15: a novel versican-cleaving proteoglycanase.
PA1-1748A was used in western blot to study the expression and processing of murine ADAMTS-15 and its potential biological functions
|Dancevic CM,Fraser FW,Smith AD,Stupka N,Ward AC,McCulloch DR||The Journal of biological chemistry (288:37267)||2013|
Pericellular versican regulates the fibroblast-myofibroblast transition: a role for ADAMTS5 protease-mediated proteolysis.
PA1-1748A was used in western blot to study regulation of the composition and volume of glycosaminoglycan-rich pericellular matrix in fibroblasts
|Hattori N,Carrino DA,Lauer ME,Vasanji A,Wylie JD,Nelson CM,Apte SS||The Journal of biological chemistry (286:34298)||2011|
Polyinosine-polycytidylic acid stimulates versican accumulation in the extracellular matrix promoting monocyte adhesion.
PA1-1748A was used in western blot to investigate the effect of polyinosine-polycytidylic acid on versican accumulation in the extracellular matrix of human lung fibroblast cultures
|Potter-Perigo S,Johnson PY,Evanko SP,Chan CK,Braun KR,Wilkinson TS,Altman LC,Wight TN||American journal of respiratory cell and molecular biology (43:109)||2010|
Synthesis and organization of hyaluronan and versican by embryonic stem cells undergoing embryoid body differentiation.
PA1-1748A was used in western blot to investigate hyaluronan and versican during embryonic stem differentiation
|Shukla S,Nair R,Rolle MW,Braun KR,Chan CK,Johnson PY,Wight TN,McDevitt TC||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (58:345)||2010|
Human TSC2-null fibroblast-like cells induce hair follicle neogenesis and hamartoma morphogenesis.
PA1-1748A was used in immunohistochemistry to study the role of tuberous sclerosis complex in hair follicle formation and tissue regeneration
|Li S,Thangapazham RL,Wang JA,Rajesh S,Kao TC,Sperling L,Moss J,Darling TN||Nature communications (2:null)||2011|
Reduced versican cleavage due to Adamts9 haploinsufficiency is associated with cardiac and aortic anomalies.
PA1-1748A was used in immunohistochemistry to study the roel of versican-degrading protease ADAMTS9 in cardiovascular development and adult homeostasis
|Kern CB,Wessels A,McGarity J,Dixon LJ,Alston E,Argraves WS,Geeting D,Nelson CM,Menick DR,Apte SS||Matrix biology : journal of the International Society for Matrix Biology (29:304)||2010|
Proteolytic cleavage of versican during limb joint development.
PA1-1748A was used in immunohistochemistry to study the proteolytic processing of versican during limb joint development
|Capehart AA||Anatomical record (Hoboken, N.J. : 2007) (293:208)||2010|
Versican proteolysis mediates myocardial regression during outflow tract development.
PA1-1748A was used in immunohistochemistry to study the role of versican proteolysis in distal myocardium loss during outflow tract remodeling
|Kern CB,Norris RA,Thompson RP,Argraves WS,Fairey SE,Reyes L,Hoffman S,Markwald RR,Mjaatvedt CH||Developmental dynamics : an official publication of the American Association of Anatomists (236:671)||2007|