Immunofluorescent analysis of Vimentin using Vimentin Monoclonal antibody (J144) (Product# MA3-745) shows staining in C6 glioma cells. Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin (Product# MA3-745) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Human rhabdosarcoma cell line JR1.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-745 detects human, rat, and mouse vimentin and has been successfully used in HELA-S3, HEK-293, rat brain, and rat kidney cells.
MA3-745 has been successfully used in Western blot, immunohistochemical and immunofluorescence procedures. By Western blot, this antibody detects an ~57 kDa protein representing vimentin from HELA-S3 cells. Immunohistochemical staining of vimentin using MA3-745 can be performed.
The MA3-745 immunogen was human rhabdosarcoma cell line JR1.
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Genetic deletion of COX-2 diminishes VEGF production in mouse retinal Müller cells.
MA3-745 was used in immunocytochemistry to investigate the role of COX2 in vascular endothelial growth factor production in retinal Muller cells
|Yanni SE,McCollum GW,Penn JS||Experimental eye research (91:34)||2010|
Protein composition of plasminogen activator inhibitor type 1-derived endothelial microparticles.
MA3-745 was used in western blot to study the protein composition of endothelial microparticles obtained from human endothelial cells stimulated with PAI-1
|Sander TL,Ou JS,Densmore JC,Kaul S,Matus I,Twigger S,Halligan B,Greene AS,Pritchard KA,Oldham KT||Shock (Augusta, Ga.) (29:504)||2008|
Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.
MA3-745 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse
|Gramolini AO,Kislinger T,Alikhani-Koopaei R,Fong V,Thompson NJ,Isserlin R,Sharma P,Oudit GY,Trivieri MG,Fagan A,Kannan A,Higgins DG,Huedig H,Hess G,Arab S,Seidman JG,Seidman CE,Frey B,Perry M,Backx PH,Liu PP,MacLennan DH,Emili A||Molecular and cellular proteomics : MCP (7:519)||2008|
Isolation of lipid droplets from cells by density gradient centrifugation.
MA3-745 was used in western blot to evaluate a density gradient centrifugation protocol for the isolation of cellular lipid droplets
|Brasaemle DL,Wolins NE||Current protocols in cell biology (Chapter 3:null)||2006|
Shiga toxin binding to globotriaosyl ceramide induces intracellular signals that mediate cytoskeleton remodeling in human renal carcinoma-derived cells.
MA3-745 was used in immunohistochemistry to investigate the role of lipid-raft-mediated cell signaling in cytoskeletal remodeling.
|Takenouchi H,Kiyokawa N,Taguchi T,Matsui J,Katagiri YU,Okita H,Okuda K,Fujimoto J||Journal of cell science (117:3911)||2004|