|Tested species reactivity||Dog, Goat, Hamster, Human, Pig, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytoskeletal vimentin extract of calf lens.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-06908 detects vimentin in human, rat, goat, canine, hamster and swine samples.
MA1-06908 has sucessfully been used in Western blotting, immunocytochemistry and immunohistochemistry (paraffin) procedures. By Western blot it detects a ~57 kDa band representing vimentin.
The MA1-06908 immunogen is cytoskeletal vimentin extract of calf lens.
Store at 4°C, or in small aliquots at -20°C.
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth.
MA1-06908 was used in western blot to study ectosome-mediated trafficking in fibroblasts
|Santi A,Caselli A,Ranaldi F,Paoli P,Mugnaioni C,Michelucci E,Cirri P||Biochimica et biophysica acta (1853:3211)||2015|