Immunofluorescent analysis of vimentin in HeLa cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at<br />room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a vimentin rabbit monoclonal antibody (Product # MA5-14564) at a dilution of 1:200 in blocking buffer overnight at 4C, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731) at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Non-human primate, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding human vimentin|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14564 targets Vimentin in IHC (P) , WB, IF and ICC applications and shows reactivity with Human, Primate, Mouse and Rat samples.
The MA5-14564 immunogen is recombinant protein encoding human vimentin.
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Cell type of origin as well as genetic alterations contribute to breast cancer phenotypes.
MA5-14564 was used in western blot to analyze the contribution of genetic alterations and cell type of origin in breast cancer phenotypes
|Bhagirath D,Zhao X,West WW,Qiu F,Band H,Band V||Oncotarget (6:9018)||2015|
Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
MA5-14564 was used in western blot to derive myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
|Zhao X,Malhotra GK,Band H,Band V||PloS one (7:null)||2012|
Clinicopathological characteristics of kidney mucinous tubular and spindle cell carcinoma.
MA5-14564 was used in immunohistochemistry - paraffin section to describe the clinicopathologically features of two cases of kidney mucinous tubular and spindle cell carcinoma
|Chen Q,Gu Y,Liu B||International journal of clinical and experimental pathology (8:1007)||2015|
A systematic analysis of commonly used antibodies in cancer diagnostics.
MA5-14564 was used in immunohistochemistry to perform a systematic study of the diagnostic power of a range of antibodies commonly used in the diagnosis of cancer
|Gremel G,Bergman J,Djureinovic D,Edqvist PH,Maindad V,Bharambe BM,Khan WA,Navani S,Elebro J,Jirström K,Hellberg D,Uhlén M,Micke P,Pontén F||Histopathology (64:293)||2014|
Molecular Magnetic Resonance Imaging of Tumors with a PTPµ Targeted Contrast Agent.
MA5-14564 was used in immunohistochemistry to develop a molecular MRI methodology utilizing a contrast agent that targets transmembrane PTPmu
|Burden-Gulley SM,Zhou Z,Craig SE,Lu ZR,Brady-Kalnay SM||Translational oncology (6:329)||2013|
Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan.
MA5-14564 was used in immunohistochemistry to study the effect of transfer from Optisol-GS to organ culture storage of the lifespan of donor corneas
|Haug K,Azqueta A,Johnsen-Soriano S,Shahdadfar A,Drolsum LK,Moe MC,Røger MT,Romero FJ,Collins AR,Nicolaissen B||Acta ophthalmologica (91:219)||2013|
Podoplanin expressing cancer associated fibroblasts are associated with unfavourable prognosis in adenocarcinoma of the esophagus.
MA5-14564 was used in immunohistochemistry to study the role and prognostic value of cancer associated fibroblasts expressing podoplanin in esophageal adenocarcinoma
|Schoppmann SF,Jesch B,Riegler MF,Maroske F,Schwameis K,Jomrich G,Birner P||Clinical and experimental metastasis (30:441)||2013|
Medulloblastoma with focal divergent/teratoid differentiation.
MA5-14564 was used in immunohistochemistry to study medulloblastoma with focal divergent/teratoid differentiation
|Majumdar K,Tyagi I,Saran RK,Sakhuja P,Sharma A||Brain tumor pathology (30:50)||2013|
Mucinous epithelial lesions in endometrial curettage material: a diagnostic challenge.
MA5-14564 was used in immunohistochemistry to study the diagnostic challenge of distinguishing benign and malignant mucinous lesions obtained at endometrial curretage
|Pavlakis K,Vrekoussis T,Messini I,Voulgaris Z,Chrysanthakis D,Yiannou P,Stofas A,Panoskaltsis T||Applied immunohistochemistry and molecular morphology : AIMM (20:607)||2012|
Residual tumor cells that drive disease relapse after chemotherapy do not have enhanced tumor initiating capacity.
MA5-14564 was used in immunohistochemistry to study the tumor-initiating capacity of the residual cells responsible for relapse following chemotherapy using non-small cell lung cancer as a model system
|Hegde GV,de la Cruz C,Eastham-Anderson J,Zheng Y,Sweet-Cordero EA,Jackson EL||PloS one (7:null)||2012|
|Non-human primate||Not Cited||
Immunohistochemical characterization of nonhuman primate ovarian sex cord-stromal tumors.
MA5-14564 was used in immunohistochemistry to study the immunohistochemistry of nonhuman primate ovarian sex cord-stromal tumors
|Durkes A,Garner M,Juan-Sallés C,Ramos-Vara J||Veterinary pathology (49:834)||2012|
|Non-human primate||Not Cited||
Gonadoblastoma in the ovaries of a lesser galago (Galago senegalensis braccatus).
MA5-14564 was used in immunohistochemistry to report on a case of gonadoblastoma in the ovaries of a lesser galago
|Irizarry Rovira AR,Lynch S,David M,Ramos Vara JA||Journal of comparative pathology (147:204)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA5-14564 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Cadherin-11, a marker of the mesenchymal phenotype, regulates glioblastoma cell migration and survival in vivo.
MA5-14564 was used in immunohistochemistry to study the expression cadherin-11 in glioblastoma cell lines and its role in cell migration and survival in xenograft models
|Kaur H,Phillips-Mason PJ,Burden-Gulley SM,Kerstetter-Fogle AE,Basilion JP,Sloan AE,Brady-Kalnay SM||Molecular cancer research : MCR (10:293)||2012|
Cotyledonoid leiomyoma of uterus: a case report.
MA5-14564 was used in immunohistochemistry to report on a case of cotyledonoid leiomyoma of uterus
|Ersöz S,Turgutalp H,Mungan S,Güvend¿ G,Güven S||Turk patoloji dergisi (27:257)||2011|
Chondroitinase ABC I-mediated enhancement of oncolytic virus spread and antitumor efficacy.
MA5-14564 was used in immunohistochemistry to investigate the effect of chondroitinase ABC on viral spread and tumor inhibition
|Dmitrieva N,Yu L,Viapiano M,Cripe TP,Chiocca EA,Glorioso JC,Kaur B||Clinical cancer research : an official journal of the American Association for Cancer Research (17:1362)||2011|
Primary myoepithelial carcinoma of the larynx: case report and review of the literature.
MA5-14564 was used in immunohistochemistry to report on a case of primary myoepithelial carcinoma of the larynx
|Yu G,Qu G,Kong L,Pan X,Wang W,Lv J||Pathology, research and practice (207:127)||2011|
Isolation and culture of adult human liver progenitor cells: in vitro differentiation to hepatocyte-like cells.
MA5-14564 was used in immunohistochemistry to investigate the culture and differentiation of human liver progenitor cells
|Gerbal-Chaloin S,Duret C,Raulet E,Navarro F,Blanc P,Ramos J,Maurel P,Daujat-Chavanieu M||Methods in molecular biology (Clifton, N.J.) (640:247)||2010|
A novel assessment of the quality of immunohistostaining overcomes the limitations of current methods.
MA5-14564 was used in immunohistochemistry to establish a straightforward procedure for objective quality evaluation of the components involved in immunohistostaining
|Eisenthal A,Trejo L,Shtabsky A,Bedny F,Brazowski E||Pathology, research and practice (204:323)||2008|
Basal-HER2 phenotype shows poorer survival than basal-like phenotype in hormone receptor-negative invasive breast cancers.
MA5-14564 was used in immunohistochemistry to study the prognostic features of basal-like breast tumors in a series of hormone receptor-negative invasive breast cancer tumors
|Liu H,Fan Q,Zhang Z,Li X,Yu H,Meng F||Human pathology (39:167)||2008|
Transplantation of human hematopoietic stem cells into ischemic and growing kidneys suggests a role in vasculogenesis but not tubulogenesis.
MA5-14564 was used in immunohistochemistry to investigate the role of human hematopoietic stem cells on vasculogenesis after cell transplant to kidney
|Dekel B,Shezen E,Even-Tov-Friedman S,Katchman H,Margalit R,Nagler A,Reisner Y||Stem cells (Dayton, Ohio) (24:1185)||2006|
Laparoscopic resection of urinary bladder paraganglioma: a case report.
MA5-14564 was used in immunohistochemistry to report on a case of aparoscopic resection of urinary bladder paraganglioma
|Dilbaz B,Bayoglu Y,Oral S,Cavusoglu D,Uluoglu O,Dilbaz S||Surgical laparoscopy, endoscopy and percutaneous techniques (16:58)||2006|
Nek8 mutation causes overexpression of galectin-1, sorcin, and vimentin and accumulation of the major urinary protein in renal cysts of jck mice.
MA5-14564 was used in immunohistochemistry to study the effect of Nek8 mutation on protein expression and accumulation in renal cysts of jck mice
|Valkova N,Yunis R,Mak SK,Kang K,Kültz D||Molecular and cellular proteomics : MCP (4:1009)||2005|
Multiple types of data are required to identify the mechanisms influencing the spatial expansion of melanoma cell colonies.
MA5-14564 was used in immunocytochemistry to use a combination of experimental data and mathematical modeling to study the spacial expansion of melanoma cells
|Treloar KK,Simpson MJ,Haridas P,Manton KJ,Leavesley DI,McElwain DL,Baker RE||BMC systems biology (7:null)||2013|
Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner.
MA5-14564 was used in immunocytochemistry to study the role of STAT3 in the mechanism by which stellate cells associated with pancreatic cancer promote the differentiation of myeloid-derived suppressor cells
|Mace TA,Ameen Z,Collins A,Wojcik S,Mair M,Young GS,Fuchs JR,Eubank TD,Frankel WL,Bekaii-Saab T,Bloomston M,Lesinski GB||Cancer research (73:3007)||2013|
Knock-down of plasminogen-activator inhibitor-1 enhances expression of E-cadherin and promotes epithelial differentiation of human pancreatic adenocarcinoma cells.
MA5-14564 was used in immunocytochemistry to study the increased E-cadherin expression and epithelial differentiation of human pancreatic carcinoma cells in response to PAI-1 deletion
|Lupu-Meiri M,Geras-Raaka E,Lupu R,Shapira H,Sandbank J,Segal L,Gershengorn MC,Oron Y||Journal of cellular physiology (227:3621)||2012|