|Flow Cytometry (Flow)||2-4µg/10^6 cells|
|Western Blot (WB)||1-3µg/ml|
|Immunocytochemistry (ICC)||See 6 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Non-human primate , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 408-591 of P18206.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with chimpanzee, Rhesus monkey, porcine, equine, rat, bovine and chicken based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Vinculin (130 kDa) is a ubiquitously expressed cytoskeletal protein that is involved in cell adhesion and migration and plays an important role in embryogenesis. Vinculin is phosphorylated at several residues sites within the tail domain. The vinculin protein consists of a globular head domain connected to an elongated tail region by a proline-rich domain. The head region contains binding sites for two cytoskeletal proteins, alpha-actinin and talin, as well as a binding site for the tail region of vinculin itself. The tail region contains binding sites for actin and paxillin. Vinculin head controls integrin dynamics whereas the tail controls the functional link between the focal adhesions and the actin cytoskeleton. Cell adhesion and migration require the coordinated formation and release of focal adhesions. During this process, the exchangeable vinculin fraction rises during movement and declines upon maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CMD1W; CMH15; epididymis luminal protein 114; Metavinculin; MV; MVCL; Vinculin
Gene Aliases: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL
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