|Tested species reactivity||Amphibian, Chicken, Fish, Hamster, Human, Mouse, Pig, Rat|
|Published species reactivity||Non-human primate, Sheep, Human, Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Vitamin D receptor protein from chicken intestine|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1mg/mL|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14614 targets Vitamin D Receptor in GS and WB applications and shows reactivity with Amphibian, Chicken, Fish, Hamster, Human, mouse, Porcine, and Rat samples.
The MA5-14614 immunogen is vitamin D receptor protein from chicken intestine.
VDR is a member of super family of steroid hormone receptors. It is known to play role in cellular growth and differentiation, calcium and phosphorus metabolism and bone remodeling. Human breast carcinomas, leukemias, and prostatic carcinoma cells express VDR, which can modify their growth and differentiation. In avians, VDR appears as a doublet of 58kDa and 60kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
Coronary artery vitamin D receptor expression and plasma concentrations of 25-hydroxyvitamin D: their association with atherosclerosis.
MA5-14614 was used in immunohistochemistry to study the relationships bewtween coronary artery vitamin D receptor expression, circulating 25-hydroxyvitamin D levels and atherosclerosis in cynomolgus monkeys
|Schnatz PF,Nudy M,O'Sullivan DM,Jiang X,Cline JM,Kaplan JR,Clarkson TB,Appt SE||Menopause (New York, N.Y.) (19:967)||2012|
Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids.
MA5-14614 was used in immunohistochemistry to study the effect of Ca2+ concentration on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of growing goats
|Sidler-Lauff K,Boos A,Kraenzlin M,Liesegang A||Journal of animal science (88:3598)||2010|
1,25 Dihydroxyvitamin D(3) receptor expression in superficial transitional cell carcinoma of the bladder: a possible prognostic factor?
MA5-14614 was used in immunohistochemistry to investigate the prognostic value of vitamin D receptors in superficial transitional cell carcinoma of the human bladder
|Sahin MO,Canda AE,Yorukoglu K,Mungan MU,Sade M,Kirkali Z||European urology (47:52)||2005|
Functionality of unliganded VDR in breast cancer cells: repressive action on CYP24 basal transcription.
MA5-14614 was used in immunocytochemistry and western blot to study the functional role of unliganded vitamin D receptor in breast cancer cells
|Alimirah F,Vaishnav A,McCormick M,Echchgadda I,Chatterjee B,Mehta RG,Peng X||Molecular and cellular biochemistry (342:143)||2010|
Chemotherapeutic effect of calcidiol derivative B3CD in a neuroblastoma xenograft model.
MA5-14614 was used in western blot to investigate the effect of bromoacetoxy-calcidiol on neuroblastoma cells and its potential therapeutic applications in vivo
|Lange TS,Zou Y,Singh RK,Kim KK,Kristjansdottir K,Sholler GL,Brard L||Chemical biology and drug design (76:164)||2010|
Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.
MA5-14614 was used in western blot to characterize mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice
|Zinser GM,McEleney K,Welsh J||Molecular and cellular endocrinology (200:67)||2003|
Induction of apoptosis by 1,25-dihydroxyvitamin D3 in MCF-7 Vitamin D3-resistant variant can be sensitized by TPA.
MA5-14614 was used in western blot to investigate the effect of TPA on the apoptosis induced by 1,25-dihydroxyvitamin D3 in MCF-7 vitamin D3-resistant variant
|Narvaez CJ,Byrne BM,Romu S,Valrance M,Welsh J||The Journal of steroid biochemistry and molecular biology (84:199)||2003|