|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Published species reactivity||Avian, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: E(395) E H S K Q Y R C L S F Q P E C S M K(413)|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-711 detects vitamin D receptor (VDR) from human, mouse and rat tissues.
PA1-711 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~53 kDa protein representing VDR in COS-7 cells transfected with the human gene. PA1-711 supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).
PA1-711 immunizing peptide corresponds to amino acid residues 395-413 from human VDR.
Recombinant human VDR (Cat. # RP-710) and human RXR beta (catalog # RP-812) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation and gel shift procedures.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expresion, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription.
The corresponding gene for the vitamin D receptor is NRI1I.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The selective vitamin D receptor agonist, elocalcitol, reduces endometriosis development in a mouse model by inhibiting peritoneal inflammation.
PA1-711 was used in immunohistochemistry to study the mechanism by which a vitamin D receptor agonist reduces endometriosis development in a mouse model
|Mariani M,Viganò P,Gentilini D,Camisa B,Caporizzo E,Di Lucia P,Monno A,Candiani M,Somigliana E,Panina-Bordignon P||Human reproduction (Oxford, England) (27:2010)||2012|
Characterization of transcriptional activation and DNA-binding functions in the hinge region of the vitamin D receptor.
PA1-711 was used in western blot to investigate the effects of hinge length on VDR activity
|Shaffer PL,McDonnell DP,Gewirth DT||Biochemistry (44:2678)||2005|
Immunochemical studies on the putative plasmalemmal receptor for 1, 25(OH)(2)D(3). I. Chick intestine.
PA1-711 was used in western blot to identify the plasmalemmal receptor for 1,25(OH)2D3 in chick
|Nemere I,Ray R,McManus W||American journal of physiology. Endocrinology and metabolism (278:E1104)||2000|