Immunofluorescent analysis of Vps20-associated 1 homolog in methanol-fixed A431 cells using a Vps20-associated 1 homolog polyclonal antibody (Product # PA5-21831) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 276 of VTA1 (Uniprot ID#Q9NP79)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 2 publications below|
PA5-21831 targets Vps20-associated 1 homolog in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-21831 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 276 of VTA1 (Uniprot ID#Q9NP79).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cytokinetic abscission is an acute G1 event.
PA5-21831 was used in immunocytochemistry to determining the timing of abscission
|Gershony O,Pe'er T,Noach-Hirsh M,Elia N,Tzur A||Cell cycle (Georgetown, Tex.) (13:3436)||2014|
|Not Applicable||Not Cited||
Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
PA5-21831 was used in immunocytochemistry to elucidate how the ESCRT machinery and the AAA+ ATPase Vps4 drive membrane scission
|Cashikar AG,Shim S,Roth R,Maldazys MR,Heuser JE,Hanson PI||eLife (3:null)||2014|