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WBP2NL Polyclonal Antibody

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Antibody Testing Data (4)

WBP2NL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

WBP2NL Antibody (22587-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human testis tissue slide using 22587-1-AP ( WBP2NL Antibody) at dilution of 1:50 (under 40x lens).

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Product Details

22587-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

WBP2NL Fusion Protein Ag18347 (1-309 aa encoded by BC022546)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAVNQSHTE NRRGALIPNG ESLLKRSPNV ELSFPQRSEG SNVFSGRKTG TLFLTSYRVI FITSCSISDP MLSFMMPFDL MTNLTVEQPV FAANFIKGTI QAAPYGGWEG QATFKLVFRN GGAIEFAQLM VKAASAAARG FPLRTLNDWF SSMGIYVITG EGNMCTPQMP CSVIVYGAPP AGYGAPPPGY GAPPAGYGAQ PVGNEGPPVG YRASPVRYGA PPLGYGAPPA GYGAPPLGYG APPLGYGTPP LGYGAPPLGY GAPPAGNEGP PAGYRASPAG SGARPHESTA AQAPENEASL PSASSSQVHS (1-309 aa encoded by BC022546)

Target Information

May play a role in meotic resumption and pronuclear formation, mediated by a WW domain-signaling pathway during fertilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CEP128 is involved in spermatogenesis in humans and mice.

Nature communications

Zhang X,Wang L,Ma Y,Wang Y,Liu H,Liu M,Qin L,Li J,Jiang C,Zhang X,Shan X,Liu Y,Li J,Li Y,Zheng R,Sun Y,Sun J,Leng X,Liang Y,Zhang F,Jiang X,Yang Y,Shen Y

Wed Mar 16 00:00:00 EDT 2022

CEP128 is involved in spermatogenesis in humans and mice.

Nature communications

Zhang X,Wang L,Ma Y,Wang Y,Liu H,Liu M,Qin L,Li J,Jiang C,Zhang X,Shan X,Liu Y,Li J,Li Y,Zheng R,Sun Y,Sun J,Leng X,Liang Y,Zhang F,Jiang X,Yang Y,Shen Y

Wed Mar 16 00:00:00 EDT 2022

Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers.

International journal of molecular sciences

Vašíček J,Baláži A,Svoradová A,Vozaf J,Dujíčková L,Makarevich AV,Bauer M,Chrenek P

Wed May 25 00:00:00 EDT 2022

Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers.

International journal of molecular sciences

Vašíček J,Baláži A,Svoradová A,Vozaf J,Dujíčková L,Makarevich AV,Bauer M,Chrenek P

Wed May 25 00:00:00 EDT 2022

A homozygous nonsense mutation of PLCZ1 cause male infertility with oocyte activation deficiency.

Journal of assisted reproduction and genetics

Wang F,Zhang J,Kong S,Li C,Zhang Z,He X,Wu H,Tang D,Zha X,Tan Q,Duan Z,Cao Y,Zhu F

Wed Apr 01 00:00:00 EDT 2020

Oligoasthenoteratozoospermic (OAT) men display altered phospholipase C ζ (PLCζ) localization and a lower percentage of sperm cells expressing PLCζ and post-acrosomal sheath WW domain-binding protein (PAWP).

Bosnian journal of basic medical sciences

Azad N,Nazarian H,Ghaffari Novin M,Masteri Farahani R,Piryaei A,Heidari MH,Abdollahpour Alitappeh M

Sun May 20 00:00:00 EDT 2018

Bosnian journal of basic medical sciences

Azad N,Nazarian H,Ghaffari Novin M,Masteri Farahani R,Piryaei A,Heidari MH,Abdollahpour Alitappeh M

Sun May 20 00:00:00 EDT 2018

PLCζ is the physiological trigger of the Ca²⁺ oscillations that induce embryogenesis in mammals but conception can occur in its absence.

Development (Cambridge, England)

Hachem A,Godwin J,Ruas M,Lee HC,Ferrer Buitrago M,Ardestani G,Bassett A,Fox S,Navarrete F,de Sutter P,Heindryckx B,Fissore R,Parrington J

Tue Aug 15 00:00:00 EDT 2017

PLCζ is the physiological trigger of the Ca²⁺ oscillations that induce embryogenesis in mammals but conception can occur in its absence.

Development (Cambridge, England)

Hachem A,Godwin J,Ruas M,Lee HC,Ferrer Buitrago M,Ardestani G,Bassett A,Fox S,Navarrete F,de Sutter P,Heindryckx B,Fissore R,Parrington J

Tue Aug 15 00:00:00 EDT 2017

Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm.

Molecular human reproduction

Kashir J,Buntwal L,Nomikos M,Calver BL,Stamatiadis P,Ashley P,Vassilakopoulou V,Sanders D,Knaggs P,Livaniou E,Bunkheila A,Swann K,Lai FA

Sun Jan 01 00:00:00 EST 2017

Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm.

Molecular human reproduction

Kashir J,Buntwal L,Nomikos M,Calver BL,Stamatiadis P,Ashley P,Vassilakopoulou V,Sanders D,Knaggs P,Livaniou E,Bunkheila A,Swann K,Lai FA

Sun Jan 01 00:00:00 EST 2017

Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.

Human molecular genetics

Escoffier J,Lee HC,Yassine S,Zouari R,Martinez G,Karaouzène T,Coutton C,Kherraf ZE,Halouani L,Triki C,Nef S,Thierry-Mieg N,Savinov SN,Fissore R,Ray PF,Arnoult C

Tue Mar 01 00:00:00 EST 2016

Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.

Human molecular genetics

Escoffier J,Lee HC,Yassine S,Zouari R,Martinez G,Karaouzène T,Coutton C,Kherraf ZE,Halouani L,Triki C,Nef S,Thierry-Mieg N,Savinov SN,Fissore R,Ray PF,Arnoult C

Tue Mar 01 00:00:00 EST 2016

Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.

Molecular human reproduction

Nomikos M,Sanders JR,Kashir J,Sanusi R,Buntwal L,Love D,Ashley P,Sanders D,Knaggs P,Bunkheila A,Swann K,Lai FA

Tue Sep 01 00:00:00 EDT 2015

Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.

Molecular human reproduction

Nomikos M,Sanders JR,Kashir J,Sanusi R,Buntwal L,Love D,Ashley P,Sanders D,Knaggs P,Bunkheila A,Swann K,Lai FA

Tue Sep 01 00:00:00 EDT 2015

Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.

Molecular human reproduction

Nomikos M,Sanders JR,Kashir J,Sanusi R,Buntwal L,Love D,Ashley P,Sanders D,Knaggs P,Bunkheila A,Swann K,Lai FA

Tue Sep 01 00:00:00 EDT 2015

Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.

Molecular human reproduction

Nomikos M,Sanders JR,Kashir J,Sanusi R,Buntwal L,Love D,Ashley P,Sanders D,Knaggs P,Bunkheila A,Swann K,Lai FA

Tue Sep 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Postacrosomal sheath WW domain-binding protein; WW domain-binding protein 2-like

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Gene Aliases: 4930521I23Rik; GRAMD7; PAWP; WBP2NL

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UniProt ID: (Human) Q8TBF0, (Mouse) Q9D529

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Entrez Gene ID: (Human) 164684, (Mouse) 74716

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Function(s)

WW domain binding

Process(es)

egg activation female pronucleus assembly male pronucleus assembly meiotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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WBP2NL Polyclonal Antibody

WBP2NL Antibody (22587-1-AP) in IHC (P)

Product Details

22587-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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