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|Tested species reactivity||Human, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified recombinant fragment of human WHSC2 (amino acids: 280-511) expressed in E. Coli.|
|Storage buffer||PBS with 0.5% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200 - 1:400|
|Immunocytochemistry (ICC)||1:200 - 1:1000|
|Immunofluorescence (IF)||1:200 - 1:1000|
|Immunohistochemistry (IHC)||1:200 - 1:1000|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17199 targets WHSC2 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Rat samples.
The MA5-17199 immunogen is purified recombinant fragment of human WHSC2 (amino acids: 280-511) expressed in E. Coli.
MA5-17199 detects WHSC2 which has a predicted molecular weight of approximately 57.3kDa.
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Negative elongation factor A; NELF-A; Wolf-Hirschhorn syndrome candidate 2 protein
NELF-A; NELFA; P/OKcl.15; WHSC2