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Search Thermo Fisher Scientific
Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
1:200-1:1,000 | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
1:200-1:1,000 | - |
Flow Cytometry (Flow) |
1/200 - 1/400 | - |
ELISA (ELISA) |
1:10,000 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Rat |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
10C8 |
Immunogen |
Purified recombinant fragment of WNT1 expressed in E. Coli. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
ascites |
Contains |
0.03% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_10984682 |
MA5-15544 targets WNT1 in FACS, IF, IHC, and WB applications and shows reactivity with Human and mouse samples.
The MA5-15544 immunogen is purified recombinant fragment of WNT1 expressed in E. Coli.
MA5-15544 detects WNT1 which has a predicted molecular weight of approximately 41kDa.
Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Proto-oncogene Int-1; Proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; Proto-oncogene Wnt-1; wingless-related MMTV integration site 1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)
Gene Aliases: BMND16; Int-1; INT1; OI15; sw; swaying; Wnt-1; WNT1
UniProt ID: (Human) P04628, (Mouse) P04426
Entrez Gene ID: (Human) 7471, (Mouse) 22408
Molecular Function:
intercellular signal molecule
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