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WNT7A/WNT7B Antibody (PA5-47430) in IHC (P)
Immunohistochemical analysis of WNT7A/WNT7B in immersion fixed paraffin-embedded sections of human endometrial cancer tissue. Samples were incubated in WNT7A/WNT7B polyclonal antibody (Product # PA5-47430) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Product Details
PA5-47430
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human Wnt-7a Leu32-Glu72, Thr216-Arg292
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610494
Product Specific Information
In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-9a, rmWnt-11, and rmWnt-16 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Wnt proteins constitute a family of secreted glycoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-7a; Protein Wnt-7b; proto-oncogene Wnt7a protein; wingless-type MMTV integration site family member 7A; wingless-type MMTV integration site family member 7B; wingless-type MMTV integration site family, member 7A; wingless-type MMTV integration site family, member 7B
Gene Aliases: WNT7A; WNT7B
UniProt ID: (Human) O00755, (Human) P56706
Entrez Gene ID: (Human) 7476, (Human) 7477
embryonic axis specification
cartilage condensation
angiogenesis
chondrocyte differentiation
neurotransmitter secretion
axonogenesis
synapse assembly
sex differentiation
asymmetric protein localization
dorsal/ventral pattern formation
positive regulation of endothelial cell migration
skeletal muscle satellite cell activation
skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration
Wnt signaling pathway
cerebellar granule cell differentiation
cell proliferation in forebrain
central nervous system vasculogenesis
establishment of cell polarity
regulation of axon diameter
response to estradiol
somatic stem cell population maintenance
embryonic forelimb morphogenesis
embryonic hindlimb morphogenesis
non-canonical Wnt signaling pathway
Wnt signaling pathway involved in wound healing, spreading of epidermal cells
synaptic vesicle recycling
embryonic digit morphogenesis
negative regulation of apoptotic process
response to estrogen
cell fate commitment
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
somatic stem cell division
stem cell development
negative regulation of neurogenesis
positive regulation of synapse assembly
palate development
positive regulation of epithelial cell proliferation involved in wound healing
oviduct development
canonical Wnt signaling pathway
dendritic spine morphogenesis
uterus morphogenesis
lens fiber cell development
cellular response to transforming growth factor beta stimulus
positive regulation of canonical Wnt signaling pathway
positive regulation of protein localization to synapse
excitatory synapse assembly
positive regulation of excitatory synapse assembly
positive regulation of excitatory postsynaptic potential
in utero embryonic development
metanephros morphogenesis
establishment or maintenance of polarity of embryonic epithelium
forebrain regionalization
neuron differentiation
lung development
oxygen homeostasis
homeostatic process
cellular metabolic process
positive regulation of osteoblast differentiation
fibroblast proliferation
embryonic organ development
synapse organization
lung morphogenesis
lung epithelium development
lobar bronchus development
trachea cartilage morphogenesis
developmental growth involved in morphogenesis
embryonic placenta morphogenesis
chorio-allantoic fusion
mammary gland epithelium development
cellular response to retinoic acid
renal inner medulla development
renal outer medulla development
outer medullary collecting duct development
inner medullary collecting duct development
stem cell proliferation
metanephric collecting duct development
metanephric epithelium development
metanephric loop of Henle development
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