OriGene

WWTR1 Monoclonal Antibody (OTI1H9), TrueMAB™

Product Image Gallery

WWTR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human prostate tissue within the normal limits using anti-WWTR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503538)

WWTR1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WWTR1 (RC204082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-WWTR1. Positive lysates LY414524 (100 µg) and LC414524 (20 µg) can be purchased separately from OriGene.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI1H9

Immunogen

Full length human recombinant protein of human WWTR1 produced in HEK293T cell.

Conjugate

Unconjugated

Form

liquid

Concentration

0.72 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor supression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1. In turn, MOB1 phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. WWTR1 regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. WWTR1 also regulates embryonic stem-cell self-renewal and promotes cell proliferation and epithelial-mesenchymal transition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: TAZ; transcriptional co-activator with PDZ-binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain-containing transcription regulator protein 1

Gene Aliases: TAZ; WWTR1

UniProt ID: (Human) Q9GZV5

Entrez Gene ID: (Human) 25937

Molecular Function: enzyme modulator kinase modulator transcription cofactor transcription factor