Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:150 | - |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Not Applicable |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI1H9 |
Immunogen |
Full length human recombinant protein of human WWTR1 produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
liquid |
Concentration |
0.72 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS with 1% BSA, 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor supression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1. In turn, MOB1 phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. WWTR1 regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. WWTR1 also regulates embryonic stem-cell self-renewal and promotes cell proliferation and epithelial-mesenchymal transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp586I1419; FLJ27004; FLJ45718; transcriptional co-activator with PDZ-binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain-containing transcription regulator protein 1
Gene Aliases: TAZ; WWTR1
UniProt ID: (Human) Q9GZV5
Entrez Gene ID: (Human) 25937
Molecular Function:
transcription cofactor
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