|Tested species reactivity||Virus|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to 15 amino acids near the carboxy terminus of the West Nile Virus core protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-20103 can be used with blocking peptide PEP-0221.
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death.
PA5-20103 was used in western blot suggest a role for ER-associated protein degradation in West Nile virus-induced cell death
|Ma H,Dang Y,Wu Y,Jia G,Anaya E,Zhang J,Abraham S,Choi JG,Shi G,Qi L,Manjunath N,Wu H||Cell reports (12:673)||2015|