Immunohistochemical analysis of WNV-Envelope protein in bird liver using a West Nile Virus envelope glycoprotein M polyclonal antibody (Product # PA5-23102) at 1 ug/ml, incubated for 30 minutes. A second antibody conjugated to biotin was applied followed with streptavidin-AKP. Antigen retrieval was performed using a protease for three minutes at room temperature with a parafilm coverslip. A West Nile Virus envelope glycoprotein M polyclonal antibody (Product # PA5-23102) was used at an initial concentration of 1 ug/ml (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute).
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (aa 8-27) of WNVM (West Nile Virus envelope glycoprotein M) protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: West Nile virus infected cell lysate.
West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.
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