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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein corresponding to residues 1-181 of human WT1.|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1: 400|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Immunoprecipitation (IP)||2-10 µg/ml lysate|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-46028 detects an epitope within residues 1-84.
Wilms and quote; tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms and quote; tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino terminus. The Wilms and quote; tumor protein binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration.
MA1-46028 was used in immunocytochemistry to study ex vivo assessment and epicardial outgrowth culture assay of epicardial-derived cell migration
|Trembley MA,Velasquez LS,Small EM||Journal of visualized experiments : JoVE (null:null)||2016|
Expression of mesothelioma-related markers in meningiomas: an immunohistochemical study.
MA1-46028 was used in immunohistochemistry - paraffin section to compare meningiomas and mesotheliomas immunohistochemically
|Abdelzaher E,Abdallah DM||BioMed research international (2014:null)||2014|
HIF-1¿, VEGF and WT-1 are protagonists in bilateral primary angiosarcoma of breast: a case report and review of literature.
MA1-46028 was used in immunohistochemistry to study the significance of the HIF-1alpha, VEGF and WT-1 in bilateral primary angiosarcoma of breast
|Al-Salam S,Balalaa N,Faour I,Akhter S,Alashari M||International journal of clinical and experimental pathology (5:247)||2012|
Mixed epithelial and stromal tumor of the kidney in a 12-year-old girl.
MA1-46028 was used in immunohistochemistry to report on a case of mixed epithelial and stromal tumor of the kidney in a 12-year-old girl
|Hara N,Kawaguchi M,Murayama S,Maruyama R,Tanikawa T,Takahashi K||Pathology international (55:670)||2005|
Wilms tumor protein; Wilms tumor protein isoform Ex4a(+); WT1; WT33
AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT1; WT33