|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal region of the human protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20993 detects Wnt1 in Human, Mouse and Rat samples.
PA1-20993 has been successfully used in Immunohistochemistry (Paraffin) and Western Blot procedures. For immunohistochemistry (paraffin) applications, boil tissue sections in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. The predicted molecular weight of PA1-20993 is 41 kDa.
PA1-20993 immunogen corresponds to Synthetic peptide corresponding to the N-terminal region of the human protein.
Wnt1 is a cysteine rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neurol tube and adult spermatids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.