|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human Wnt-1|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-2.0 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||0.1-0.2ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections.
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
Wnt1, formerly known as int1, is a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer. Wnt1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a protooncogene implicated in carcinomas induced by the mouse mammary tumour virus (MMTV). Wnt1 belongs to a family of Wnt genes involved in the dorsoventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.