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XDH Antibody (CF812777) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XDH (RC219402, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-XDH. (1:5. Positive lysates LY400135 (100 µg) and LC400135 (20 µg) can be purchased separately from OriGene.
Product Details
CF812777
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI1C10
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-280 of human XDH produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
XDH belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. This protein can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Xanthine dehydrogenase/oxidase; xanthine oxidase; xanthine oxidoreductase; xdh antibody; xdha antibody; xor antibody
Gene Aliases: XDH; XDHA; XO; XOR; Xox-1; Xox1
UniProt ID: (Human) P47989, (Rat) P22985, (Mouse) Q00519
Entrez Gene ID: (Human) 7498, (Rat) 497811, (Mouse) 22436
xanthine dehydrogenase activity
xanthine oxidase activity
iron ion binding
electron carrier activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the aldehyde or oxo group of donors
protein homodimerization activity
molybdopterin cofactor binding
flavin adenine dinucleotide binding
2 iron, 2 sulfur cluster binding
UDP-N-acetylmuramate dehydrogenase activity
oxidoreductase activity
catalytic activity
protein binding
molybdenum ion binding
metal ion binding
iron-sulfur cluster binding
oxidoreductase
metabolite interconversion enzyme
negative regulation of protein phosphorylation
negative regulation of endothelial cell proliferation
purine nucleotide catabolic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
lactation
xanthine catabolic process
negative regulation of gene expression
negative regulation of endothelial cell differentiation
negative regulation of protein kinase B signaling
oxidation-reduction process
positive regulation of p38MAPK cascade
negative regulation of vascular endothelial growth factor signaling pathway
positive regulation of reactive oxygen species metabolic process
negative regulation of vasculogenesis
response to aluminum ion
regulation of epithelial cell differentiation
bone resorption
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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