OriGene

XDH Monoclonal Antibody (OTI1C10), TrueMAB™

View all (13) XDH antibodies

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XDH Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XDH (RC219402, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-XDH. (1:5. Positive lysates LY400135 (100 µg) and LC400135 (20 µg) can be purchased separately from OriGene.

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:500

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI1C10

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-280 of human XDH produced in E.coli.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

XDH belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. This protein can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: XAN1; Xanthine dehydrogenase; Xanthine dehydrogenase/oxidase; Xanthine oxidase; Xanthine oxidoreductase; XD; XO; XOR

Gene Aliases: XDH; XDHA; XO; XOR

UniProt ID: (Human) P47989

Entrez Gene ID: (Human) 7498

Molecular Function: dehydrogenase oxidase oxidoreductase

negative regulation of protein phosphorylation negative regulation of endothelial cell proliferation purine nucleotide catabolic process activation of cysteine-type endopeptidase activity involved in apoptotic process lactation xanthine catabolic process negative regulation of gene expression negative regulation of endothelial cell differentiation negative regulation of protein kinase B signaling oxidation-reduction process positive regulation of p38MAPK cascade negative regulation of vascular endothelial growth factor signaling pathway positive regulation of reactive oxygen species metabolic process negative regulation of vasculogenesis

Process(es)

xanthine dehydrogenase activity xanthine oxidase activity iron ion binding electron carrier activity oxidoreductase activity, acting on CH-OH group of donors oxidoreductase activity, acting on the aldehyde or oxo group of donors protein homodimerization activity molybdopterin cofactor binding flavin adenine dinucleotide binding 2 iron, 2 sulfur cluster binding