Immunofluorescent analysis of XPC in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a XPC monoclonal antibody (Product # MA1-23328). Green: Primary antibody at a dilution of 1:100. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant XPC protein purified from E. coli|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/100|
|Western Blot (WB)||1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23328 detects XPC in human samples.
MA1-23328 has been successfully used in immunofluorescence, immunohistochemistry (paraffin) and Western blot procedures.
The MA1-23328 immunogen is recombinant XPC protein purified from E. coli.
Human XPC (Xeroderma pigmentosum group C) is a member of a family of proteins that has been shown to be involved in the repair of DNA via the nucleotide excision repair (NER) pathway. Specifically, XPC is believed to be a part of a heteromeric protein complex that is involved in the recognition of the DNA lesions during global genomic repair but not transcription-coupled repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.