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|Tested species reactivity||Human|
|Published species reactivity||Hamster, Human|
|Host / Isotype||Mouse|
|Immunogen||Recombinant human XPF protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12054 targets XPF in IHC (P) and WB applications and shows reactivity with Human samples.
The MA5-12054 immunogen is recombinant human XPF protein.
The structure-specific ERCC1/XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV-induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross-links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Comparison of ERCC1/XPF genetic variation, mRNA and protein levels in women with advanced stage ovarian cancer treated with intraperitoneal platinum.
MA5-12054 was used in western blot to study potential correlations between ERCC1 and XPF expression, genotype and clinical outcome in advanced epithelial ovarian cancer patients undergoing platinum chemotherapy
|Deloia JA,Bhagwat NR,Darcy KM,Strange M,Tian C,Nuttall K,Krivak TC,Niedernhofer LJ||Gynecologic oncology (126:448)||2012|
Role of interaction of XPF with RPA in nucleotide excision repair.
MA5-12054 was used in western blot to study the role of the interaction of RPA and XPF during nucleotide excision repair
|Fisher LA,Bessho M,Wakasugi M,Matsunaga T,Bessho T||Journal of molecular biology (413:337)||2011|
Poly(ADP-ribose) polymerase and XPF-ERCC1 participate in distinct pathways for the repair of topoisomerase I-induced DNA damage in mammalian cells.
MA5-12054 was used in western blot to investigate the diffrent role of PARP and XPF-ERCC1 in mammalian DNA repair
|Zhang YW,Regairaz M,Seiler JA,Agama KK,Doroshow JH,Pommier Y||Nucleic acids research (39:3607)||2011|
Downregulation of XPF-ERCC1 enhances cisplatin efficacy in cancer cells.
MA5-12054 was used in western blot to study the effect of cisplatin on XPF-ERCC1 in cancer cells
|Arora S,Kothandapani A,Tillison K,Kalman-Maltese V,Patrick SM||DNA repair (9:745)||2010|
Processing of a psoralen DNA interstrand cross-link by XPF-ERCC1 complex in vitro.
MA5-12054 was used in western blot to study the molecular mechanism by which the XPF-ERCC1 complex processes a DNA substarate containing a single psoralen interstrand cross-link
|Fisher LA,Bessho M,Bessho T||The Journal of biological chemistry (283:1275)||2008|
Molecular characterization of spontaneous mesenchymal stem cell transformation.
MA5-12054 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation
|Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A||PloS one (3:null)||2008|
The knock-down of ERCC1 but not of XPF causes multinucleation.
MA5-12054 was used in immunocytochemistry to study the role of ERCC1 in cell cycle progression and multinucleation
|Rageul J,Frëmin C,Ezan F,Baffet G,Langouët S||DNA repair (10:978)||2011|
Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue.
MA5-12054 was used in immunocytochemistry, immunoprecipitation, and western blot to evaluate a panel of antibodies for the immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue
|Bhagwat NR,Roginskaya VY,Acquafondata MB,Dhir R,Wood RD,Niedernhofer LJ||Cancer research (69:6831)||2009|
Mutations of the SLX4 gene in Fanconi anemia.
MA5-12054 was used in immunoprecipitation to study SLX4 mutations in two patients with Fanconi anemia
|Kim Y,Lach FP,Desetty R,Hanenberg H,Auerbach AD,Smogorzewska A||Nature genetics (43:142)||2011|
Human XPF controls TRF2 and telomere length maintenance through distinctive mechanisms.
MA5-12054 was used in immunoprecipitation to study the distinct mechanisms by which XPF-ERCC1 regulates TRF2 function and maintenance of telomere length
|Wu Y,Mitchell TR,Zhu XD||Mechanisms of ageing and development (129:602)||2008|
complementation group F; complementing defective; DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair; excision-repair, complementing defective, in Chinese hamster; in Chinese hamster; xeroderma pigmentosum; xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F
ERCC11; ERCC4; FANCQ; RAD1; XFEPS; XPF