|Tested species reactivity||Human|
|Published species reactivity||Hamster, Human, Not Applicable|
|Host / Isotype||Mouse|
|Immunogen||Recombinant human XPF protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12054 targets XPF in IHC (P) and WB applications and shows reactivity with Human samples.
The MA5-12054 immunogen is recombinant human XPF protein.
The structure-specific ERCC1/XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV-induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross-links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phase II study of cetuximab in combination with cisplatin and radiation in unresectable, locally advanced head and neck squamous cell carcinoma: Eastern cooperative oncology group trial E3303.
MA5-12054 was used in immunohistochemistry to characterize unresectable, locally advanced head and neck squamous cell carcinoma in an Easter cooperative oncology group trial E3303 phase II study of cetuximab in combination with radiation and cisplatin
|Egloff AM,Lee JW,Langer CJ,Quon H,Vaezi A,Grandis JR,Seethala RR,Wang L,Shin DM,Argiris A,Yang D,Mehra R,Ridge JA,Patel UA,Burtness BA,Forastiere AA||Clinical cancer research : an official journal of the American Association for Cancer Research (20:5041)||2014|
Comparison of ERCC1/XPF genetic variation, mRNA and protein levels in women with advanced stage ovarian cancer treated with intraperitoneal platinum.
MA5-12054 was used in western blot to study potential correlations between ERCC1 and XPF expression, genotype and clinical outcome in advanced epithelial ovarian cancer patients undergoing platinum chemotherapy
|Deloia JA,Bhagwat NR,Darcy KM,Strange M,Tian C,Nuttall K,Krivak TC,Niedernhofer LJ||Gynecologic oncology (126:448)||2012|
Role of interaction of XPF with RPA in nucleotide excision repair.
MA5-12054 was used in western blot to study the role of the interaction of RPA and XPF during nucleotide excision repair
|Fisher LA,Bessho M,Wakasugi M,Matsunaga T,Bessho T||Journal of molecular biology (413:337)||2011|
Poly(ADP-ribose) polymerase and XPF-ERCC1 participate in distinct pathways for the repair of topoisomerase I-induced DNA damage in mammalian cells.
MA5-12054 was used in western blot to investigate the diffrent role of PARP and XPF-ERCC1 in mammalian DNA repair
|Zhang YW,Regairaz M,Seiler JA,Agama KK,Doroshow JH,Pommier Y||Nucleic acids research (39:3607)||2011|
Downregulation of XPF-ERCC1 enhances cisplatin efficacy in cancer cells.
MA5-12054 was used in western blot to study the effect of cisplatin on XPF-ERCC1 in cancer cells
|Arora S,Kothandapani A,Tillison K,Kalman-Maltese V,Patrick SM||DNA repair (9:745)||2010|
Processing of a psoralen DNA interstrand cross-link by XPF-ERCC1 complex in vitro.
MA5-12054 was used in western blot to study the molecular mechanism by which the XPF-ERCC1 complex processes a DNA substarate containing a single psoralen interstrand cross-link
|Fisher LA,Bessho M,Bessho T||The Journal of biological chemistry (283:1275)||2008|
Molecular characterization of spontaneous mesenchymal stem cell transformation.
MA5-12054 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation
|Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A||PloS one (3:null)||2008|
The knock-down of ERCC1 but not of XPF causes multinucleation.
MA5-12054 was used in immunocytochemistry to study the role of ERCC1 in cell cycle progression and multinucleation
|Rageul J,Frëmin C,Ezan F,Baffet G,Langouët S||DNA repair (10:978)||2011|
Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue.
MA5-12054 was used in immunocytochemistry, immunoprecipitation, and western blot to evaluate a panel of antibodies for the immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue
|Bhagwat NR,Roginskaya VY,Acquafondata MB,Dhir R,Wood RD,Niedernhofer LJ||Cancer research (69:6831)||2009|
Mutations of the SLX4 gene in Fanconi anemia.
MA5-12054 was used in immunoprecipitation to study SLX4 mutations in two patients with Fanconi anemia
|Kim Y,Lach FP,Desetty R,Hanenberg H,Auerbach AD,Smogorzewska A||Nature genetics (43:142)||2011|
Human XPF controls TRF2 and telomere length maintenance through distinctive mechanisms.
MA5-12054 was used in immunoprecipitation to study the distinct mechanisms by which XPF-ERCC1 regulates TRF2 function and maintenance of telomere length
|Wu Y,Mitchell TR,Zhu XD||Mechanisms of ageing and development (129:602)||2008|