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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant human XPF protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12060 targets XPF in WB applications and shows reactivity with Human samples.
The MA5-12060 immunogen is recombinant human XPF protein.
The structure-specific ERCC1/XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV-induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross-links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Xeroderma pigmentosum group F protein binds to Eg5 and is required for proper mitosis: implications for XP-F and XFE.
MA5-12060 was used in western blot to study the association between Eg5 and Xeroderma pigmentosum group F protein and the significance for mitosis and the pathogenesis of XP-F and XFE
|Tan LJ,Saijo M,Kuraoka I,Narita T,Takahata C,Iwai S,Tanaka K||Genes to cells : devoted to molecular & cellular mechanisms (17:173)||2012|
Delayed c-Fos activation in human cells triggers XPF induction and an adaptive response to UVC-induced DNA damage and cytotoxicity.
MA5-12060 was used in western blot to study the role of delayed c-Fos activation in the adaptive response to UVC-induced DNA damage and cytotoxicity
|Tomicic MT,Reischmann P,Rasenberger B,Meise R,Kaina B,Christmann M||Cellular and molecular life sciences : CMLS (68:1785)||2011|
gamma-H2AX formation in response to interstrand crosslinks requires XPF in human cells.
MA5-12060 was used in western blot to examine the role of XPF
|Mogi S,Oh DH||DNA repair (5:731)||2006|
Phosphorylation of XPB helicase regulates TFIIH nucleotide excision repair activity.
MA5-12060 was used in western blot to study the role of XPB helicase phosphorylation in the regulation of the nucleotide excision repair activity of TFIIH
|Coin F,Auriol J,Tapias A,Clivio P,Vermeulen W,Egly JM||The EMBO journal (23:4835)||2004|
Repair of laser-localized DNA interstrand cross-links in G1 phase mammalian cells.
MA5-12060 was used in immunocytochemistry to study the repair of laser-localized DNA interstrand cross-links in G1 phase mammalian cells
|Muniandy PA,Thapa D,Thazhathveetil AK,Liu ST,Seidman MM||The Journal of biological chemistry (284:27908)||2009|
Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step.
MA5-12060 was used in blocking or activating experiment to study the recruitment of repair factors during nucleotide excision repair and the initiating role played by XPG
|Mocquet V,Lainé JP,Riedl T,Yajin Z,Lee MY,Egly JM||The EMBO journal (27:155)||2008|
complementation group F; complementing defective; DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair; excision-repair, complementing defective, in Chinese hamster; in Chinese hamster; xeroderma pigmentosum; xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F
ERCC11; ERCC4; FANCQ; RAD1; XFEPS; XPF