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Product Details
UM500020CF
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
UMAB20
Immunogen
Full length human recombinant protein of human XPF produced in HEK293 cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA excision repair protein ERCC-4; DNA repair endonuclease subunit; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; Ercc4 protein-like; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; Xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F
Gene Aliases: AI606920; ERCC11; ERCC4; FANCQ; RAD1; RGD1560340; XFEPS; XPF
UniProt ID: (Human) Q92889, (Mouse) Q9QZD4
Entrez Gene ID: (Human) 2072, (Dog) 479842, (Mouse) 50505, (Rat) 304719
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
damaged DNA binding
single-stranded DNA binding
endodeoxyribonuclease activity
protein binding
protein C-terminus binding
structure-specific DNA binding
protein N-terminus binding
nucleic acid binding
DNA binding
nuclease activity
endonuclease activity
hydrolase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
DNA metabolism protein
endodeoxyribonuclease
nucleic acid metabolism protein
resolution of meiotic recombination intermediates
telomere maintenance
double-strand break repair via homologous recombination
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
response to UV
UV protection
negative regulation of telomere maintenance
nucleotide-excision repair, DNA incision
cellular response to UV
interstrand cross-link repair
global genome nucleotide-excision repair
nucleotide-excision repair involved in interstrand cross-link repair
DNA metabolic process
cellular response to DNA damage stimulus
DNA catabolic process, endonucleolytic
obsolete telomere maintenance via telomere shortening
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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