|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human XRCC1 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2 - 4 µg/ml|
|Western Blot (WB)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HT29, LS174T, HeLa cells or normal testis.
XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cadmium treatment suppresses DNA polymerase ¿ catalytic subunit gene expression by acting on the p53 and Sp1 regulatory axis.
MA1-12640 was used in western blot to report a Cd-mediated impairment of BER pathway at sub-lethal doses
|Antoniali G,Marcuzzi F,Casarano E,Tell G||DNA repair (35:90)||2015|
Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability.
MA1-12640 was used in western blot to test if base excision repair-deficient cells are a source of pre-cancerous cells
|Markkanen E,Fischer R,Ledentcova M,Kessler BM,Dianov GL||Nucleic acids research (43:3667)||2015|
|Not Applicable||Not Cited||
ATM prevents DSB formation by coordinating SSB repair and cell cycle progression.
MA1-12640 was used in immunocytochemistry and western blot to study the coordination of SSB repair and cell cycle progression where ATM prevents DSB formation
|Khoronenkova SV,Dianov GL||Proceedings of the National Academy of Sciences of the United States of America (112:3997)||2015|
|Not Applicable||Not Cited||
Association between polymorphisms of XRCC1, p53 and MDR1 genes, the expression of their protein products and prognostic significance in human breast cancer.
MA1-12640 was used in immunohistochemistry - paraffin section to assess the relationship between XRCC1, p53, and MDR1 protein and their prognostic values for breast cancer
|Rybárová S,Vecanová J,Hodorová I,Mihalik J,¿i¿máriková M,Moj¿i¿ J,Solár P,Benický M,Adamkov M,Mirossay L||Medical science monitor : international medical journal of experimental and clinical research (17:BR354)||2011|