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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human XRCC1 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2 - 4 µg/ml|
|Western Blot (WB)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control for this product is HT29, LS174T, HeLa cells or normal testis.
XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability.
MA1-12640 was used in western blot to test if base excision repair-deficient cells are a source of pre-cancerous cells
|Markkanen E,Fischer R,Ledentcova M,Kessler BM,Dianov GL||Nucleic acids research (43:3667)||2015|
RCC; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross-complementing protein 1